四种培养基对大丽轮枝菌V991b及突变体的影响

The effect of four kinds of medium types on Verticillium dahliae V991b and its mutant

为了解黄萎病菌V991b及其突变体V991w的生物学特性和致病力,调查了不同条件培养基对2种病原菌的生长及致病力的影响。结果表明,2种病原菌的生长曲线基本呈线性。V991b在PDA培养基上的生长速率与其他培养基之间差异显著,其他3种培养基之间差异不显著;V991w在PDA和PCA培养基上的生长速率之间差异不显著,与其他两种培养基之间差异显著。接种于PDA培养基15 d后,V991b与V991w的菌落生长直径之间无显著差异。经查氏液体培养基摇菌后接种棉苗,25 d时V991b和V991w的致病力分别为56.63和23.23,而经查氏-棉叶组织液体培养基摇菌后接种棉苗,其病情指数明显降低,接种25 d时V991b和V991w的病指仅为12.12和7.32。棉叶组织培养基对大丽轮枝菌微菌核的直径生长无显著影响,但对微菌核的数量和菌丝生长有明显的抑制作用。

In order to understand the biological characteristics and pathogenicity of Verticillium dahilae V991b and its mutant V991w,the effects of different conditioned medium on the growth and pathogenicity of the two pathogens were investigated.The results showed that the growth curve of two strains on different types of medium was linear basically.The growth rate of V991b on PDA was significantly different from other types of medium.And the growth rate of V991w on PDA and PCA medium was significantly different from other two types of medium.After cultiviated 15 days on PDA medium,there was no significantly difference between the colony diameter of V991b and that of V991w.During amplified the fungi in Czapek’s liquid medium and then inoculated the cotton seedling,the pathoge⁃nicity of V991b and V991w was quite different.The disease index on 25 days of inoculated was 56.63 and 23.23,respectively.However,the disease index of cotton seedlings inoculated with Czapek’s-cotton leaf tissue liquid medium was significantly reduced,and the dis⁃ease index of V991b and V991w at 25 d inoculation was only 12.12 and 7.32.The plant tissue culture medium had no significant effect on the diameter of the microsclerotia,but it had significant inhibitory effect on the number of microsclerotia and the growth of hypha.