不同氨基聚糖对巨噬细胞极化的影响

Effects of different glycosaminoglycans on macrophage polarization

目的探讨不同氨基聚糖——透明质酸(HA)和硫酸软骨素(CS)对巨噬细胞极化的作用,旨在选出能减少炎症反应及组织损伤的细胞移植支架材料。方法将小鼠骨髓源性巨噬细胞分别培养于HA/CS水凝胶中,在不同时间(0、4、24、48、72 h)收集标本,实时qPCR检测白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、诱导性一氧化氮合酶(iNOS)和精氨酸酶-1(Arg-1)表达水平。结果与0 h比较,48 h HA水凝胶中巨噬细胞TNF-α和iNOS表达增加(P<0.05)。与0 h比较,48 h CS水凝胶中巨噬细胞Arg-1表达增加(P<0.05)。结论HA可使巨噬细胞极化为具有促炎作用的M1型,而CS使巨噬细胞极化为具有抗炎作用的M2型。作为细胞移植支架材料,与HA比较,CS可以减少炎症损伤、保护组织。

Objective We examined the effects of different glycosaminoglycans(hyaluronic acid and chondroitin sulfate)on macrophage polarization,thinking on the design of a better hydrogel scaffold to attenuate tissue inflammation and injury.Methods We cultured bone marrow derived macrophage in hyaluronic acid/chondroitin sulfate-containing hydrogel for 0,4,24,48,and 72 h,and then conducted RT-qPCR to evaluate the expression of interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),and arginase-1(Arg-1).Results Comparing with the 0 h time-point,the mRNA expression of TNF-αand iNOS increased from the 48 h time-point onwards in the context of the hyaluronic acid hydrogel(P<0.05);the mRNA expression of Arg-1 also increased after 48 h in the context of the chondroitin sulfate hydrogel(P<0.05).Conclusion Hyaluronic acid probably polarizes macrophages into an M1 phenotype,promotes inflammation,while chondroitin sulfate polarizes macrophages into an anti-inflammatory M2 phenotype.Therefore,chondroitin sulfate is a better scaffold material than hyaluronic acid to suppress inflammation and attenuate injury.