加味丹参饮治疗心肌梗死的网络药理学研究

Study on network pharmacology of Jiawei Danshen Decoction in treatment of myocardial infarction

目的基于网络药理学的方法,分析加味丹参饮对于治疗心肌梗死的潜在作用机制。方法借助TCMSP、Drugbank、Pubchem、Swiss Target Prediction等数据库及参考文献获取加味丹参饮的有效成分及靶标。通过HPO、OMIM平台检索心肌梗死疾病相关的靶标,并借助STRING数据库构建靶蛋白相互作用网络(PPI),获得关键靶标。使用Cytoscape3.6.0软件对加味丹参饮-心肌梗死疾病进行网络可视化,并构建"加味丹参饮-化学成分-靶点"、"疾病-靶点"、"加味丹参饮-相关化学成分-疾病-关键靶点"网络图,使用DAVID数据库对关键靶点进行GO生物过程和KEGG代谢通路分析。结果从加味丹参饮中共筛选出94个有效成分,148个与心肌梗死相关的作用靶点,其中有效成分与疾病对应的相同靶点21个,其中包含血管内皮生长因子A(VEGFA)、酪氨酸激酶2(JAK2)、肿瘤坏死因子(TNF)等。经GO和KEGG通路富集分析,靶点主要涉及G蛋白偶联受体信号通路、调节细胞增殖、RNA聚合酶II转录因子活性等功能过程,可能通过调节PI3K-Akt信号通路、微小RNA-癌症相关通路、AMPK信号通路、VEGF信号通路、JAK-STAT信号通路等多条代谢通路来发挥加味丹参饮治疗心肌梗死的作用。结论通过建立靶点网络图,可从多方位阐明复方加味丹参饮治疗心肌梗死多成分、多靶点的机制,可进一步为深入研究加味丹参饮治疗心肌梗死相关疾病机制提供理论依据。

Objective Based on the method of network pharmacology,the potential mechanism of Jiawei Danshen Decoction in the treatment of myocardial infarction was analyzed.Methods Tcmsp,drugbank,PubChem,Swiss target prediction and other databases and references were used to obtain the effective components and targets of Jiawei Danshen Decoction.The targets related to myocardial infarction were searched by HPO and OMIM platforms,and the PPI was constructed with STRING database to obtain the key targets.Using the software of Cytoscape 3.6.0,the network visualization of Jiawei Danshen Decoction-myocardial infarction disease was carried out,and the network diagram of"Jiawei Danshen Decoction-chemical composition target","disease-target"and"Jiawei Danshen Decoction-related chemical components–disease-key target"was constructed.DAVID database was used to analyze the GO biological process and KEGG metabolic pathway of key targets.Results A total of 94 active components,and 148 targets related to myocardial infarction were screened out from Jiawei Danshen Decoction.Among them,there were 21 targets corresponding to the disease,VEGFA,JAK2,TNF,etc.After enrichment analysis of GO and KEGG pathways,the target proteins mainly involved in G protein coupled receptor signaling pathway,cell proliferation regulation,RNA polymerase II transcription factor activity and other functional processes,it may play a role in treating myocardial infarction by regulating PI3 K Akt signaling pathway,microRNA cancer related pathway,AMPK signaling pathway,VEGF signaling pathway,and JAK-STAT signaling pathway.Conclusion Through the establishment of target network map,we can clarify the mechanism of multicomponent and multi-target of Jiawei Danshen Decoction in treatment of myocardial infarction,and provide theoretical basis for further research on the mechanism of Jiawei Danshen Decoction in treating myocardial infarction related diseases.