基于网络药理学探讨枣仁安神颗粒治疗失眠的作用机制

Anti-insomnia effect of Zaoren Anshen Granules based on network pharmacology

目的基于网络药理学探讨枣仁安神颗粒治疗失眠的作用机制。方法通过查阅相关文献,挖掘枣仁安神颗粒的入血成分,并采用反向分子对接,预测入血成分的相关靶点,与失眠靶点进行交集映射,获得其治疗失眠的作用靶点。利用蛋白互作筛选核心靶点,并进行分子对接验证。基于共有靶点,采用功能注释生物信息学芯片进行分析,得到枣仁安神颗粒治疗失眠的关键通路。结果方中3味中药酸枣仁、丹参、北五味子共得到29个入血成分和184个潜在靶点,其中19个成分、60个靶点和36条通路与失眠极为密切,主要涉及神经活性配体-受体相互作用、色氨酸代谢、5-羟色胺能突触、GABA能突触等相关分子环节。结论方中3味中药以作用靶点为媒介,连接入血成分及相关通路,从而发挥抗失眠的生物学效应,体现了中药多成分、多靶点,多途径协同起效的作用特点。

Objective The mechanism of the treatment of insomnia by Zaoren Anshen Granules was discussed based on the network pharmacology.Methods By consulting the relevant literatures,the blood components of Zaoren anshen granules were excavated.Reverse molecular docking was used to predict the relevant targets of chemical components,Potential targets of Zaoren anshen granules were obtained by mapping with targets of insomnia.Subsequently,protein-protein interactions were used to screen core targets and molecular docking validation was performed.Based on the above-mentioned common targets,functional annotation bioinformatics chips were used to analyze the key pathways for the treatment of insomnia by Zaoren anshen granules,and to explore the possible mechanism of treatmenting insomnia.Results Three traditional Chinese medicines,29 blood components and 184 potential targets were obtained,of which 19 components and 60 targets and 36 pathways were related to insomnia,mainly related to neuroactive ligand-receptor interaction,tryptophan metabolism,serotonin synapses,GABA ergic synapses and other related molecular links.Conclusion The targets were used as a medium to connect blood components and related pathways,thus exerting the biological effect of insomnia,reflecting the multi-components,multi-targets and multi-pathways synergistic action of traditional Chinese medicine.