摘要
以核桃、枇杷、茶树等植物幼嫩器官的匀浆上清液作为多酚氧化酶来源,通过液相质谱联用仪检测茶黄素,比较不同植物多酚氧化酶催化茶多酚生成茶黄素的活力。试验结果表明,核桃幼嫩花絮来源的多酚氧化酶生成茶黄素的量是梨、枇杷和茶树等幼叶来源的3~10倍,而核桃幼叶和云南山茶幼叶的匀浆上清液几乎不能催化茶多酚生成茶黄素,梨幼叶和枇杷幼叶的相对酶活力高于四种茶树的幼叶。上述植物多酚氧化酶生成的茶黄素中,以茶黄素-3,3’-双没食子酸酯的含量最高。
The homogenate supernatant of young organs of walnut,loquat,tea and other plants was used as the source of polyphenol oxidase.Theaflavins was detected by liquid chromatography mass spectrometry(LC-MS),and the activity of different plant polyphenol oxidase to catalyze theaflavins synthesis from tea polyphenols was compared.The results showed that the amount of theaflavins produced by polyphenol oxidase from walnut flowers was 3-10 times as much as that of young leaves of pear,loquat and tea.While the homogenate supernatant of young leaves of walnut and Yunnan camellia could hardly catalyze theaflavins synthesis from tea polyphenols.The relative activity of young leaves of pear and loquat was higher than that of four kinds of tea young leaves.The content of theaflavin-3,3’-digallate was the highest in theaflavins produced by these plant polyphenol oxidases.
作者
曾俊
ZENG Jun(State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;University of Chinese Academy of Science,Beijing 100049,China)
出处
《茶叶通讯》
北大核心
2020年第3期443-449,共7页
Journal of Tea Communication
基金
植物化学与西部植物资源持续利用国家重点实验室和云南省天然药物化学重点实验室项目。
