三种荚蒾中香豆素提取工艺及含量比较分析研究

Study on extraction technology and content of coumarin from three varieties of Viburnum

以皱叶荚蒾(Viburnum rhytidophyllum)、球核荚蒾(Viburnum propinquum)和鸡树条荚蒾(Viburnum sargentii)为原料,选取乙醇浓度、提取时间、提取温度和料液比为因素,吸光度为指标进行单因素试验,选取适当因素水平进行正交试验,并采用高效液相色谱法(HPLC)进行试验,采用优化后的工艺进行提取,比较3种荚蒾(Viburnum dilatatum)中香豆素的提取率。结果表明,3种荚蒾中均含有香豆素,其中,皱叶荚蒾中香豆素的含量最高,球核荚蒾次之,鸡树条荚蒾最少;皱叶荚蒾和球核荚蒾香豆素最佳提取条件为乙醇浓度55%、提取时间60 min、提取温度80℃、料液比1∶25(g∶mL);鸡树条荚蒾香豆素最佳提取条件为乙醇浓度55%、提取时间80 min、提取温度70℃、料液比1∶25(g∶mL)。不同品种荚蒾中香豆素的提取工艺和所提取的香豆素含量均存在差异。

The raw materials are Viburnum rhytidophyllum,Viburnum propinquum and Viburnum sargentii.The single factor experi⁃ment was carried out by selecting ethanol concentration,extraction time,extraction temperature and ratio of material to liquid as fac⁃tors,absorbance as index,and then orthogonal test analysis was carried out at appropriate factor level and HPLC was used to carry out the experiment.The yield of coumarin from three varieties of Viburnum was compared by the optimized extraction process.The results showed that coumarin was found in all three kinds of Viburnum,Viburnum rhytidophyllum was the highest,Viburnum propinquum was the second,and Viburnum sargentii was the least.The optimum conditions of Viburnum rhytidophyllum and Viburnum propinquum were as follows:The concentration of ethanol was 55%,the extraction time was 60 min,the extraction temperature was 80℃,and the ratio of material to liquid was 1∶25.The optimum conditions of Viburnum sargentii were as follows:The concentration of ethanol was 55%,the extraction time was 80 min,the extraction temperature was 70℃,and the ratio of material to liquid was 1∶25.The content of coumarin was different in the extraction process of different varieties of Viburnum.