应用iTRAQ技术快速鉴定稻米品质差异蛋白的方法分析

Rapid identification of differential protein related to rice quality by iTRAQ

以鄂中5号和桂朝2号为材料,应用iTRAQ技术快速鉴定稻米品质差异蛋白,从而根据差异蛋白快速和有效地筛选到与水稻(Oryza sativa L.)品质相关联的候选基因。结果表明,鄂中5号、桂朝2号共鉴定出水稻显著差异蛋白61个,其中上调蛋白21个,下调蛋白40个。该技术可广泛应用于水稻品质性状的研究和开发并且结合转基因技术培育高档优质水稻品种。

Using Ezhong No.5 and Guichao No.2 as materials,the iTRAQ technology was used to quickly identify rice quality differ⁃ential proteins,so that candidate genes related to rice quality can be rapidly and effectively screened based on the differential proteins.The results showed that Ezhong No.5 and Guichao No.2 identified a total of 61 significantly different proteins in rice,including 21 up-regulated proteins and 40 down-regulated proteins.This technology can be widely used in the research and development of rice quality traits and combined with transgenic technology to cultivate high-quality rice varieties.