非生物胁迫蒙古黄芪基因组的MSAP分析

Genome MSAP Analysis of Astragalus mongholicus under Abiotic Stresses

本研究初探了珍稀中药材蒙古黄芪的抗逆能力与其DNA甲基化程度之间的关系。挑选内蒙古等地区常有的非生物胁迫因素——干旱与高盐,以蒙古黄芪培养7 d苗龄幼苗为材料,采用聚乙二醇(PEG-6000)及NaCl分别模拟不同程度的干旱及高盐胁迫,DNA提取后进行基因组的甲基化(MSAP)分析,分别研究其DNA甲基化程度的变化,并对PEG胁迫浓度与DNA甲基化程度进行了回归分析;结果与对照组相比,干旱作用下,DNA甲基化程度随PEG-6000浓度增加而降低,从大到小在56.6%至26.1%之间变化;高盐作用下,DNA甲基化程度随盐浓度增加而降低,从大到小在51.2%至35.3%之间变化。说明蒙古黄芪DNA甲基化的MSAP比率随PEG-6000与盐浓度增加均呈下降趋势,与胁迫呈显著负相关。内蒙古等地区蒙古黄芪生长区域由于缺乏科学管理,不能很好利用当地气候条件、管理粗放等因素,影响蒙古黄芪的正常生长。本研究旨在非生物胁迫因素——干旱和高盐与蒙古黄芪之间的内在关联,旨在找出应答胁迫因素的基因,希望能够通过外界调控,提高蒙古黄芪的产率。

The relationship between the adverse reaction ability and the degree of DNA methylation of mongolia astragalus was preliminarily explored in this paper.Drought and high salinity,two common abiotic stress factors in Inner Mongolia and other regions,were selected in the study and the 7-day-old seedlings of Mongolia Astragalus were cultured as materials.Polyethylene glycol(PEG-6000)and NaCl were used to simulate different degrees of drought and high salinity stress.The DNA was extracted and analyzed by methylation sensitive amplification polymorphism(MSAP).The changes of DNA methylation in astragalus membranaceus genome were studied,and the relationship between PEG stress concentration and DNA methylation was analyzed by regression analysis.The results showed that:Compared with the control group,the degree of DNA methylation varied from 56.6%to 26.1%with the increase of PEG-6000 concentration and from 51.2%to 35.3%with the increase of salt concentration.They reveal that MSAP ratio of DNA methylation of mongolia astragalus decreased with the increase of PEG-6000 and salt concentration,there was a significantly negative correlation between MSAP and abiotic stresses.In Inner Mongolia and other regions,the growth of Astragalus mongolica is affected by the lack of scientific management,the inability to make good use of local climate conditions,extensive management and other factors.The purpose of this experiment is to find out the genes that respond to abiotic stress factors-drought and high salinity,and to improve the yield of astragalus membranaceus through external regulation.