MSC与PRP对膝关节软骨再生的宏观和组织学影响

Macroscopic and Histological Effects of Bone Marrow Mesenchymal Stem Cells and Platelet-rich Plasma Pulp on Cartilage Regeneration in Knee Joint

为探讨骨关节炎实验模型中骨髓间充质干细胞(MSC)和富含血小板血浆(PRP)对膝关节软骨再生的宏观和组织学影响。本研究将40只家兔随机分为4组:对照组、富血小板血浆组、自体MSC未分化组和自体MSC分化为软骨细胞组。使用胶原酶溶液用于诱导骨关节炎,并且在骨关节炎诱导后6周对每组施用治疗。治疗60 d后,对动物实施安乐死,并对关节面进行肉眼和组织学评估。本研究评估了MSC的脂肪形成,软骨形成和成骨分化潜能。同时肉眼观察和组织学检查显示MSC治疗组的组织修复得到改善。然而,MSC分化的和未分化的软骨细胞之间没有发现差异。本研究发现源自脂肪组织和富含血小板的血浆的MSC与实验性骨关节炎期间关节软骨再生存在相关性。该结果为进一步研究其他动物模型和临床试验提供了基础,并突出了MSCs和PRPs在再生医学中的潜力。

In order to evaluate the macroscopic and histological effects of bone marrow mesenchymal stem cells(MSC)and platelet-rich plasma(PRP)on knee joint cartilage regeneration in an experimental model of osteoarthritis.Forty rabbits were randomly divided into 4 groups:control group,platelet-rich plasma group,autologous MSC undifferentiated group and autologous MSC differentiated into chondrocyte group.A collagenase solution was used for the induction of osteoarthritis,and treatment was administered to each group 6 weeks after the induction of osteoarthritis.After 60 days of treatment,the animals were euthanized and the joint surfaces were evaluated visually and histologically.This research evaluated the potential of MSC for adipogenesis,cartilage formation and osteogenic differentiation.At the same time,visual inspection and histological examination showed that the tissue repair of the MSC treatment group was improved.However,no difference was found between MSC differentiated and undifferentiated chondrocytes.This study found a correlation between MSCs derived from adipose tissue and platelet-rich plasma and the beneficial effects of articular cartilage regeneration during experimental osteoarthritis.The results of this study provide the basis for further study of other animal models and clinical trials,and highlight the potential of MSCs and PRPs in regenerative medicine.