颈交感神经阻滞对糖尿病大鼠认知功能、线粒体呼吸功能和炎症因子的影响

Effects of Stellate Ganglion Block on Cognitive Function, Mitochondrial Respiratory Function and Inflammatory Factors in Diabetic Rats

为了探讨颈交感神经阻滞对糖尿病大鼠认知功能、线粒体呼吸功能和炎症因子的影响。本研究将60只SD大鼠随机分为对照组、模型组和颈交感神经阻滞组,每组20只。采用链脲佐菌素(STZ)诱导糖尿病大鼠模型,并应用颈交感神经阻滞治疗大鼠。Morris水迷宫实验显示,模型组大鼠的逃避潜伏期显著高于对照组(34.54 s vs 13.49 s),颈交感神经阻滞组大鼠的逃避潜伏期(25.76 s)显著低于模型组。模型组大鼠的穿越平台次数显著低于对照组(0.61次vs 5.22次),但颈交感神经阻滞组大鼠的穿越平台次数(1.78次)显著大于模型组(p<0.05)。检测各组大鼠的线粒体呼吸功能指标后发现,颈交感神经阻滞组大鼠的Ⅲ态呼吸(R3)和呼吸控制率(RCR)均显著高于模型组(p<0.05)。采用HE染色评估大鼠海马CA1区病理变化,发现颈交感神经阻滞组大鼠的海马CA1区病理变化较模型组明显减轻,神经细胞受损程度明显降低。通过RT-PCR和Western blotting检测各组大鼠海马组织TLR4、TNF-α、IL-1β和IL-6 m RNA和蛋白的表达,发现颈交感神经阻滞组大鼠海马组织中的TLR4、TNF-α、IL-1β和IL-6 m RNA表达水平均显著低于模型组(p<0.05)。表明颈交感神经阻滞可有效改善大鼠的认知功能和线粒体呼吸功能,并抑制中枢系统的炎症反应。

To investigate the effects of stellate ganglion block on cognitive function,mitochondrial respiratory function and inflammatory factors in diabetic rats.In this study,60 SD rats were randomly divided into control group,model group and stellate ganglion block group,with 20 rats in each group.Diabetic rat models were induced with streptozotocin(STZ)and treated with stellate ganglion block.Morris water maze experiments showed that the escape latency of the model group was significantly higher than that of the control group(34.54 s vs 13.49 s),and the escape latency of the rats in the stellate ganglion block(25.76 s)was significantly lower than that of the model group.The number of crossing platforms in the model group was significantly lower than that in the control group(0.61 vs 5.22),but the number of crossing platforms in the stellate ganglion block(1.78)was significantly greater than that in the model group(p<0.05).After detecting the mitochondrial respiratory function of each group,theⅢstate respiration(R3)and respiratory control rate(RCR)of the stellate ganglion block were significantly higher than those of the model group(p<0.05).HE staining was used to evaluate the pathological changes of hippocampal CA1 area in rats.It was found that the pathological changes of hippocampal CA1 area in rats with stellate ganglion block were significantly reduced compared with the model group,and the degree of neuronal damage was significantly reduced.The expressions of TLR4,TNF-α,IL-1βand IL-6 mRNA and protein in hippocampus of each group were detected by RT-PCR and Western blotting.The expression levels of TLR4,TNF-α,IL-1βand IL-6 mRNA in hippocampus of rats with stellate ganglion block were significantly lower than those in the model group(p<0.05).It indicates that stellate ganglion block can effectively improve cognitive function and mitochondrial respiratory function in rats,and inhibit the inflammatory response of the central nervous system.