脑得生片的HPLC指纹图谱研究

Study on HPLC fingerprint of Naodesheng tablets

目的:利用HPLC法建立脑得生片的对照指纹图谱。方法:以葛根素、3′-羟基葛根素、3′-甲氧基葛根素和大豆苷为指标,采用phenomenex C18色谱柱(250 mm×4.6 mm,5μm),以甲醇(A)-0.1%甲酸(B)为流动相,梯度洗脱(0~30 min,5%A;30~60 min,5%A→15%A;60~170 min,15%A→20%A;170~210 min,20%A→40%A),流速1.0 ml/min,检测波长为250 nm,柱温30℃。采用Chempattern化学计量软件对结果进行处理分析。结果:建立脑得生片的指纹图谱,共确定脑得生片HPLC指纹图谱6个共有峰,通过与混合对照品比较,指认其中的4个指标成分。结论:所建立指纹图谱专属性强,能够为脑得生片的掺伪控制提供科学依据。

Objective:To establish a HPLC fingerprint for Naodesheng Tablets. Methods: Puerarin,3′-hydroxypuerarrin,3′-methoxypuerarin,Daidzin and Daidzein were used as indicators. HPLC analysis was performed on a phenomenex C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.1% formic acid aqueous solution with gradient elution(0~30 min,5%A;30~60 min,5%A→15%A;60~170 min,15%A→20%A;170~210 min,20%A→40%A). The flow rate was 1.0 m L/min,and the detection wavelength was 250 nm with the column temperature was 30 ℃. Chemometrics software Chempattern was employed to analyze the data. Results: The common mode of the fingerprint was set up with 6 common peaks,and 5 of them were identified by comparison with the reference. Conclusion: The established fingerprint is highly specific and can provide a scientific basis for the quality control of Naodesheng tablets.