低温胁迫下仁用杏花转录组测序分析

RNA Sequencing Analysis of Flowers of Kernel Apricot(Prunus armeniaca L.) under Low Temperature Stress

为了研究仁用杏花的转录组信息以及寻找室温(CK)和低温处理的仁用杏花的差异表达基因,本试验以抗寒性强的仁用杏品种‘围选1号’的花为试验材料,采用高通量转录组测序技术对室温(CK)和低温处理的‘围选1号’的花进行转录组测序。测序结果显示,共获得Transcript和Unigene分别为116957条和31360条,其中N50分别为2311 nt和2262 nt。使用BLAST软件将Transcript序列与NR、GO、Swiss-Prot、KOG和KEGG数据库比对,注释到各数据库的序列分别为84231条、12955条、60914条、50978条和7185条。并对CK和低温处理的样品之间表达量方差最大的前50个基因做聚类分析,结果分为两大类,第一类有12个基因,在CK中表达量较高,在低温处理中表达量较低;第二类有38个基因,在低温处理中表达量较高,在CK中表达量较低。此研究结果获得了仁用杏花的转录信息,并寻找到了室温(CK)和低温处理的仁用杏花的差异表达基因,为今后采取基因工程手段培育抗寒的仁用杏新品种提供了基因资源。

In order to study transcr iptome information of kernel apricot flowers and to search for differentially expressed genes between room temperature(CK)and low temperature of kernel apricot flowers,flowers of kernel apricot’Weixuan No.1’with strong cold resistance were used as experimental materials in this experiment.RNA sequencing was performed on the flowers of’Weixuan No.1’at room temperature(CK)and low temperature by high-throughput sequencing.Sequencing results showed that transcript and Unigene were 116957 and 31360,respectively,of which N50 were 2311 nt and 2262 nt,respectively.The BLAST software was used to compare transcript sequences with the NR,Swiss-Prot,GO,KOG,and KEGG databases,and transcript sequences that were annotated to each database were 84231,60914,12955,50978,and 7185,respectively.The cluster analysis was performed on the top 50 genes with the largest difference in expression between CK and low temperature treatment.The results were divided into two categories.The first class had 12 genes,which were highly expressed in CK,but lowly expressed in low temperature treatment.The second class had 38 genes,which had higher expression in low temperature than in CK.The results of this study obtained the transcriptional information of kernel apricot flowers,and found the differentially expressed genes of kernel apricot flowers at room temperature(CK)and low temperature treatment.The results will provide genetic resources for breeding new kernel apricot varieties with cold resistance by genetic engineering in the future.