HPLC测定北芪五加颗粒中紫丁香苷的含量

Content Determination of Syringin in Beiqi Wujia Granules by HPLC

为了建立测定北芪五加颗粒中紫丁香苷含量的高效液相色谱法(HPLC)。采用phenomenex 00G-4435-E0 C18(250 mm×4.60 mm×5μm)色谱柱;以甲醇-水(20∶80)为流动相;检测波长为265 nm;流速为1 mL/min。结果显示,紫丁香苷在5.05~80.75μg/mL范围内有较好的线性关系(R2=0.9999),平均回收率为100.43%(RSD=1.18%,n=6)。表明本检测方法简单,结果准确,重现性好,可用于北芪五加颗粒的质量控制。

To establish an HPLC method for the determination of syringin content of Beiqi Wujia Granules,all separations were carried out on a phenomenex 00G-4435-E0 C18 column(250 mm×4.60 mm×5μm).The mobile phase was methanol-water(20∶80),the detection wavelength was 265 nm,and the flow rate was 1 mL/min.Syringin showed a good liner relationship in the range of 5.05μg/ml^80.75μg/ml(R 2=0.9999).The average recovery was 100.43%(RSD=1.18%,n=6).The established method is sample,accurate,and reproducible,which can be used for the quality control of Beiqi Wujia Granules.