不同强度净风、风沙流吹袭对黑果枸杞叶片生理指标的影响

Effects of net-wind and wind-sand flow on the physiology of Lycium ruthenicum

以黑果枸杞(Lycium ruthenicum)一年实生苗为材料,研究0(CK)、6、9、12、15 m·s^–15个风速下净风和风沙流吹袭对黑果枸杞叶片丙二醛(MDA)含量、膜透性、酶活性、渗透调节物质含量的影响,探索黑果枸杞对净风和风沙流胁迫的生理响应机制。结果表明:净风处理下,MDA含量、超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、可溶性蛋白含量、可溶性糖含量均逐渐降低,膜透性、过氧化物酶(POD)活性均逐渐增大,脯氨酸含量先上升后下降。风沙流处理下,MDA含量、POD活性、可溶性糖含量均呈先上升后下降的变化趋势,其余指标的变化趋势与净风处理一致。净风和风沙流处理均对黑果枸杞叶片造成一定程度的伤害,黑果枸杞受胁迫时通过POD活性的提高增强保护作用;净风胁迫下,主要通过脯氨酸含量的提高增强渗透调节作用;风沙流胁迫下,主要通过脯氨酸和可溶性糖含量的提高增强渗透调节作用。

The present study investigated the effects of net-wind and wind-sand flow on the malondialdehyde content,membrane permeability,protective enzyme activity,and osmoregulation substance content of Lycium ruthenicum leaves with wind speeds of 0(CK),6,9,12,and 15 m·s^–1.The net-wind treatment,malondialdehyde content,superoxide dismutase(SOD)activity,catalase(CAT)activity,soluble protein content,and soluble sugar content decreased,whereas membrane permeability and peroxidase(POD)activity increased,and proline content increased first and then decreased.Under the wind-sand flow treatment,malondialdehyde content,POD activity,and soluble sugar content all increased first and then decreased,whereas the trends of other indicators were identical to those of the net-wind treatment.Both the net-wind and wind-sand flow treatments damaged the leaves,and this stress caused L.ruthenicum to increase POD activity and to enhance osmotic regulation,either by eliciting increases in proline content(net-wind flow stress)or by eliciting increases in both proline and soluble sugar contents(wind-sand flow stress).