加味大承气汤活血攻下法治疗肠梗阻的网络药理学研究

Study on Network Pharmacology of Modified Dachengqi Decoction in the Treatment of Intestinal Obstruction

目的:基于网络药理学的方法探讨加味大承气汤治疗肠梗阻(Intestinal obstruction)的作用机制。方法:通过中药系统药理学数据库(TCMSP)来获得加味大承气汤所含中药的主要化学成分,并收集其相对应的靶点;肠梗阻的疾病靶点来源于GeneCards、DrugBank、Home-OMIM-NCBI数据库。使用Draw Venn Diagrams工具获得交集基因。结合Cytoscape3.7.1对所得的交集基因进行PPI分析、GO与KEGG通路富集分析。结果:共获得加味大承气汤活性化学成分121个,药物靶点1478个;利用GeneCards、DrugBank、Home-OMIM-NCBI数据库获得疾病靶点147个;通过Draw Venn Diagrams工具获得交集基因24个交集基因;通过PPI蛋白作用网络及网络拓扑分析,EGFR(表皮生长因子受体)在网络连接程度较高;通过GO富集分析发现:主要参与了对营养水平的反应、平滑肌细胞增殖的调控、平滑肌细胞增殖等生物学过程;通过KEGG通路富集分析发现:主要涉及PI3K-Akt信号通路、JAK-STAT信号通路以及HIF-1信号通路等。结论:加味大承气汤活血攻下法治疗肠梗阻是多靶点、多途径的,本次研究为进一步研究其作用机制提供依据。

Objective Based on the method of network pharmacology,to explore the mechanism of Jiawei Dachengqi Decoction in the treatment of intestinal obstruction.Methods the main chemical components of traditional Chinese medicine in Jiawei Dachengqi Decoction were obtained by tcmsp,and the corresponding targets were collected;the disease targets of intestinal obstruction were from genecards,drugbank and home OMIM NCBI.Use the draw Venn diagrams tool to get the intersection gene.PPI analysis and enrichment analysis of go and KEGG pathway were carried out in combination with Cytoscape 3.7.1.Results a total of 121 active chemical components and 1478 drug targets were obtained in Jiawei Dachengqi Decoction,147 disease targets were obtained by genecards,dragbank and home OMIM NCBI database,and 147 disease targets were obtained by draw Venn Through the analysis of PPI protein action network and network topology,EGFR(epidermal growth factor receptor)has a high degree of network connectivity;through the analysis of go enrichment,it is found that EGFR mainly participates in the response to nutrition level,the regulation of smooth muscle cell proliferation,smooth muscle cell proliferation and other biological processes;through the analysis of KEGG pathway enrichment,it is found that EGFR(epidermal growth factor receptor)has a high degree of network connectivity:it mainly involves PI3K Akt signal path,JAK-STAT signal path and HIF-1 signal path.Conclusion Jiawei Dachengqi decoction is a multi-target and multi-channel method for the treatment of intestinal obstruction.This study provides a basis for further study of its mechanism.