雌激素β受体拮抗剂在老年小鼠骨质疏松性骨折中作用的实验研究

Effect of ER-βantagonist on osteoporosis fracture in elderly mice

目的探讨雌激素β受体拮抗剂在老年骨质疏松小鼠股骨骨折修复中的影响。方法采用高选择性雌激素β受体拮抗剂(PHTPP)阻断雌激素β受体的老年骨质疏松小鼠股骨骨折模型形成实验组,未使用拮抗剂的老年骨质疏松小鼠股骨骨折模型作为对照组,在不同时间点采用组织学检查、动态骨组织形态计量学分析及生物力学测试等方法观察骨折愈合情况,实验组与对照组进行比较。结果术后2周、4周,实验组骨痂总面积、软骨骨痂面积、软骨骨痂比值均高于对照组,差异均有统计学意义(均P<0.05);术后4周、6周,实验组极限负荷、断裂能、刚度均高于对照组,差异均有统计学意义(均P<0.05)。结论雌激素β受体拮抗剂通过促进早期血管形成,中期的软骨内化骨和晚期的机械力学性能等作用,能够提高老年骨质疏松小鼠股骨开放骨折的修复能力,说明了高选择性雌激素β受体拮抗剂(PHTPP)可能作为潜在的新药来治疗老年女性骨折。

Objective To explore the influence of estrogen receptor-beta(ER-β)antagonist on the femoral fracture repair of elderly osteoporosis mice.Methods The elderly osteoporosis mice who took highly selective antagonist of ER-β(PHTPP)were set an experimental group;and the elderly osteoporosis mice who did not took highly selective antagonist of ER-βwere set as a control group.The results of histological examination,dynamic histomorphometric analysis,and mechanical test in different preset time points were compared between the two groups.Results 2 and 4 weeks after the operation,total osteotylus area,gristle osteotylus area,and gristle osteotylus ratio were higher in the experimental group than in the control group(all P<0.05).4 and 6 weeks after the operation,the limit load,fracture energy,and rigidity were higher in the experimental group than in the control group(all P<0.05).Conclusion ER-βantagonist can promote the repair capability of elderly osteoporosis mice for their open femur fracture by simulating vascularization in early stage,accelerating endochondrostosis in midterm stage,and enhancing mechanic capability in later stage,which means PHPPT is a potential candidate of new medicine for the elderly female fractures.