生鲜畜禽肉中沙门氏菌全基因组分析与分子溯源

Whole genome analysis and molecular traceability study of Salmonellain fresh livestock and poultry meat

依据GB 4789.4—2016对从长沙市生鲜市场随机采集的生鲜猪肉、鸡肉进行检验。结果显示:有20份生鲜猪肉样品检出沙门氏菌,检出率为83.3%;有20份生鲜鸡肉样品检出沙门氏菌,检出率为74.1%;总检出率为78.4%。通过对其中40株沙门氏菌进行全基因组测序与分子溯源研究,结果表明:20株猪源性沙门氏菌与20株鸡源性沙门氏菌按分子溯源分析可分为5个明显不同的聚类,清晰反映了沙门氏菌的污染源分布及交叉污染情况,实现了精准溯源;其中发现了15个致病基因,从基因层面阐明了沙门氏菌可能存在的致病基因与致病机制,提示了沙门氏菌潜在的致病风险;试验发现了15个耐药基因,从基因层面阐明了沙门氏菌可能存在的耐药基因与耐药机制。

In order to investigate the contamination status of Sal monellain fresh pork and fresh chicken,and research its whole genome characteristics and molecular traceability,the laboratory randomly collected 24 fresh pork and 27 fresh chicken from the fresh market in Changsha,were inspected according to"National Food Safety Standard Food microbiological examination:Salmonella"GB 4789.4—2016.The results showed that in 24 randomly selected fresh pork,20 samples were detected,with the detection rate of 83.3%;in 27 randomly selected fresh chicken,20 samples were detected,and the detected rate is 74.1%;the total detection rate was 78.4%.The results showed that the fresh pork and fresh chicken sold on the fresh market in Changsha were seriously contaminated by Salmonella,which had great risk for food safety.40 of them were analyzed by whole genome sequencing and molecular traceability analysis.The results showed that 20 kinds of pig-derived Salmonella strains and 20 kinds of chickenderived Salmonella strains could be divided into 5 clusters,and clearly reflected the distribution of pollution sources and crosscontamination of Salmonella,achieving precise traceability.15 pathogenic genes were found,which clarified the possible pathogenic genes and pathogenic mechanisms on the genetic level,and suggested the potential risk of Salmonella.The research on the pathogenic mechanism of related diseases provided guidance for diseases control.15 genes involved in drug-resistance were found,which clarified the possible drug-resistant genes and the relative mechanisms on the genetic level,providing important data for the control of highly drug-resistant strains.