马王堆古尸蛋白质及蛋白质组学的分析与鉴定

Analysis and Identification of Protein and Proteomics of Mawangdui Ancient Corpse

[目的]分析马王堆古尸的部分组织中存留蛋白质及蛋白质组学。[方法]采用免疫组化方法检测马王堆尸体的皮肤、肌肉和皮下组织的存留蛋白,并对肌肉组织的存留蛋白进行质谱分析和鉴定。[结果]古尸皮肤、肌肉及皮下组织未观察到细胞结构,免疫组化染色方法均未检测到组蛋白和肌动蛋白。质谱分析结果显示:在肌肉组织中共鉴定出216种蛋白。[结论]采用质谱法检测和鉴定马王堆古尸中那些丢失了细胞结构的组织中的蛋白质,其可能在马王堆女尸的全身整体保存和器官组织大体形态维持中发挥着独特的作用。

[Objective]To analyze the remaining proteins and proteomics in some tissues of Mawangdui ancient corpse.[Methods]The remaining proteins in skin.muscle and subcutaneous tissues of Mawangdui corpse were detected by immunohistochemistry.and the remaining proteins in muscle tissues were analyzed and identified by mass spect rometry.[Results]No cell structure was observed in the skin.muscle and subcuta-neous tissue of the corpse.Histone and actin were not detected by immunohistochemistry.The results of mass spectrometry showed that 216 proteins were identified in muscle tissue.[Conclusion]It may play a unique role in the overall preservation of Mawangdui female corpse and the maintenance of organ morphology by mass spectrometry.