miR-125在宫颈癌组织中的表达及其临床意义

Expression and Clinical Significance of mir-125 in Cervical Cancer

[目的]探讨miR-125在宫颈癌组织中的表达及其临床意义。[方法]收集110例宫颈癌组织及其对应的癌旁正常组织标本,采用RT-PCR检测miR-125水平,分析共表达与宫颈癌患者病理特征及预后的相关性。采用免疫组织化学法检测TRAF6蛋白在癌组织及癌旁组织标本中的表达。[结果]miR-125在宫颈癌组织中的表达明显低于癌旁正常组织(P<0.05);miR-125的表达水平与肿瘤的分化程度、临床分期及淋巴结转移相关(P<0.05);miR-125高表达的中位生存期高于低表达者(P<0.05)。[结论]miR-125在宫颈癌中低表达,与宫颈癌的分化程度、临床分期、淋巴结转移、宫旁浸润及患者预后相关,提示miR-125可作为宫颈癌临床诊疗的新靶点及预后评价的指标。

[Objective]To study the expression of miR-125 and its elinical significance in cervical cancer tis-sues.[Methods]The expression of mir-125 was detected by RT-PCR in 110 cases of cervical cancer and its corre-sponding normal tissues.The correlation between the expression of mir-125 and the pa thological characteristies and.prognosis of patients with cervical cancer was analyzed,The expression of TRAF6 protein in cancer tissues and ad-jacent tissues was detected by immunohistochemistry.[Results]The expression of mir 125 in cervical cancer was significantly lower than that in adjacent normal tissues(P<0.05);the expression level of mir-125 was correlated with tumor differentiation,clinical stage and lymph node metastasis(P<0.05);the median survival time of high.expression of mir 125 was higher than that of low expression patients(P<0.05).[Conclusion]The low expres-sion of mir-125 in cervical cancer is related 10 the degree of differentiation,clinical stage,lymph node metastasis,parauterine invasion and prognosis of cervical cancer,suggesting that mir-125 can be used as a new target for clini-cal diagnosis and treatment of cervical cancer and an indicator for prognosis evaluation.