环丙沙星对TRAIL诱导的肺癌细胞凋亡作用机制研究

Effect and Mechanism of Ciprofloxacin(CIP)on Apoptosis of Lung Cancer Cells Induced by TRAIL

[目的]探讨环丙沙星(eiprofloxacin,CIP)对肿瘤坏死因子相关凋亡诱导配体(tumor necrosis factor-associated apoprosis inducing ligand,TRAIL)诱导的肺癌细胞凋亡效果和机制。[方法]人腺癌A549细胞分为空白对照组(未处理)、CIP组(培养基中加入CIP 100 μg/mL)、TRAIL组(培养基中加入TRAIL 50 ng/mL)、联合组(培养基中加入CIP 100 μg/mL.和TRAIL 50 ng/mL),采用台盼蓝染色计算细胞活力,采用Westerb blot法检测死亡受体4(DR4)、死亡受体5(DR5),CCAAT/增强子结合蛋白同源蛋白(CHOP)表达。[结果]空白对照组和CIP组A549细胞活力相比较差异无统计学意义(P>0.05).TRAIL组和联合组细胞活力低于空白对照组与CIP组<P<0.05>.且联合组A549细胞活力显著低于TRAIL组(P<0.05)。24 h、48 h时,TRAIL组CHOP、DR4.DR5蛋白相对表达水平均显著高于空白对照组和CIP组(P<0.05),且联合组CHOP,DR4,DR5相对表达量均显著高于TRAIL组(P<0.05)。[结论]TRAIL联合CIP可上调A549细胞DR4、DR5表达,降低A549细胞活力,增加A549细胞凋亡水平;CHOP可能在CIP诱导的DR5上调和肺腺癌A549细胞凋亡中发挥重要作用,值得临床进一步研究。

[Objective]To investigate the efect and mechanism of ciprofloxacin(CIP)on apoptosis of lung canc-er cells induced by(tumor necrosis factor-associated apoptosis inducing ligand,TRAIL)[Methods]Human adenocar-einoma A549 cells were divided into blank control group(untreated),CIP group(CIP 100μg/mL in medium),trail group(trail 50 ng/mL,in medium)and combination group(CIP 100 μg/mL and trail 50 ng/mL in medium);Trypan blue staining was used to calculate cell viability;The expression of death receptor 4(DR4),death receptor 5(DR5)and CCAAT/enhancer binding protein homologous protein(CHOP)were detected by Western blot.[Results]There was no significant difference between the blank control group and the CIP group in A549 cell viability(P>0.05).The cell viability of the TRAIL,group and the CIP group was lower than that of blank the control group and the CIP group(P<0.05),and the A549 cell viability of the combined group was significantly lower than that of the trail group(P<0.05).At 24 h and 48 h,the relative expression levels of CHOP,DR4 and DR5 in the TRAIL.group were significantly higher than those in the blank control group and the CIP group(P<0.05).and the relative ex-pression levels of CHOP,DR4 and DR5 in the combined group were significantly higher than those in the TRAIL group(P<0.05).[Conclusion]TRAIL combined with CIP can up regulate the expression of DR4 and DR5 in A549 cells,reduce the viability of A549 cells,and increase the apoptosis level of A549 cells;CHOP may play an important role in CIP induced DR5 up regulation and lung adenocarcinoma A549 cell apoptosis,which is worthy of further clinical research.