114例疑似COVID-19患者呼吸道病毒感染分析

Analysis of respiratory virus infection in 114 suspected COVID-19 patients

目的分析114例COVID-19疑似患者呼吸道病毒感染情况,探讨多种病毒同时检测在疫情防控中的价值。方法利用Real Time RT-PCR技术检测发热门诊COVID-19疑似患者SARS-CoV-2核酸,利用基因芯片恒温扩增技术检测SARS-CoV-2核酸阴性患者其他常见的18种呼吸道病毒。结果114例COVID-19疑似患者SARS-CoV-2核酸均为阴性,有21例感染了非其它呼吸道病毒,感染率达18.42%,21例患者中共检测出10种呼吸道病毒,包括冠状病毒NL63/229型、呼吸道合胞病毒、柯萨奇病毒A16型、乙型流感病毒、人副流感病毒1型、人副流感病毒3型、人偏肺病毒、甲型流感病毒、甲型流感病毒季节性H3亚型、肠道病毒/鼻病毒。其中乙型流感病毒感染患者最多,有6例,呼吸道合胞病毒有5例。有3例患者同时感染2种病毒:呼吸道合胞病毒与柯萨奇病毒A16型混合感染、冠状病毒NL63/229型与人副流感病毒1型混合感染、甲型流感病毒与甲型流感病毒季节性H3亚型混合感染。结论在应对本次SARS-CoV-2疫情中,针对COVID-19疑似患者中,要注意鉴别SARS-CoV-2与其它呼吸道病毒,及时有效地排除疑似病例。

To explore the value of simultaneous detection of multiple viruses in epidemic prevention and control of COVID-19.To analyze respiratory virus infection of 114 suspected COVID-19 patients,real-time RT-PCR was used to detect SARS-CoV-2 nucleic acid.At the same time,the thermostatic amplification was used to detect other 18 respiratory virus.As results,the nucleic acid of 114 suspected COVID-19 patients was negative,and 21 of them were infected with non-other respiratory viruses,with an infection rate of 18.42%.A total of 10 respiratory viruses were detected in 21 cases,including coronavirus NL63/229E,respiratory syncytial virus,human coxsackie virus A16,influenza B virus,human parainfluenza virus type 1,human parainfluenza virus type 3,human metapneumovirus,influenza A virus,seasonal influenza a virus subtype H3,and enterovirus/rhinovirus.There were 6 cases of influenza B virus infection and 5 cases of respiratory syncytial virus.Three patients were co-infected with two viruses:respiratory syncytial virus mixed with coxsackie virus A16,coronavirus NL63/229E mixed with human parainfluenza virus 1,and influenza A virus mixed with influenza A virus seasonal H3 subtype.In conclusion,in response to the SARS-CoV-2 epidemic,attention should be paid to the identification of SARS-CoV-2 and other respiratory viruses in suspected COVID-19 patients,so as to effectively exclude suspected cases.