槲皮苷对体外大鼠PI3K-Akt信号通路与神经营养作用影响的相关性研究

Correlation Study on Influence of Quercitrin Hydrate on PI3K-Akt Signaling pathway and Nerve-nutrient Effect in Vitro in Rats

目的:探究槲皮苷对体外大鼠PI3K-Akt信号通路与神经营养作用影响的相关性。方法:采用大鼠体外培养皮质神经元,实验分为正常对照组、LY294002(10μmol/L)组、槲皮苷(100μmol/L)组及LY294002(10μmol/L)+槲皮苷(100μmol/L)组,用MTT法检测不同浓度的LY294002(5, 10, 20μmol/L)对皮质神经元的毒性作用,观察其对槲皮苷促进皮质神经元的存活及突起生长是否有拮抗作用。结果:不同浓度的LY294002对皮质神经元的存活率没有影响,不具有细胞毒作用;LY294002(10μmol/L)+槲皮苷(100μmol/L)组有突起神经元数目和平均突起长度与槲皮苷(100μmol/L)组相比均显著性减少,而与溶媒对照组相比均无显著性差异。结论:LY294002可以阻断槲皮苷神经的营养作用,说明槲皮苷的神经营养作用是通过激活下游的PI3K-Akt信号转导通路而发挥作用。

Objective: To study the correlation influence of quercitrin hydrate on PI3 K-Akt signaling pathway and nerve-nutrient effect in vitro in rats. Methods: The newborn rat cerebral cortex neurons were cultured in vitro. The experiment was divided into normal control group, LY294002(10 μmol/L) group, quercetin(100 μmol/L) group and LY294002(10 μmol/L)+quercetin(100 μmol/L) group. MTT assay was used to investigate the effect of LY294002 on the neuronal viability. Taking advantage of the availability of the LY294002(a specific inhibitor of PI3 K upstream of Akt), to explore whether it would block the neurotrophic effects of quercitrin hydrate on neurite outgrowth and survival. Results: MTT assay showed that quercitrin hydrate at the concentration of 5 μmol/L, 10 μmol/L and 20 μmol/L had no effect on the survival rate of cortical neurons and had no cytotoxic effect. Compared with the quercetin(100 μmol/L) group, the LY294002(10 μmol/L)+quercetin(100 μmol/L) group reduced the number of survival neurons with neurites and the average length of neurites. Conclusion: LY294002 could block the neurotrophic effect of quercitrin, indicating that the neurotrophic effect of quercitrin played a role by activating the downstream PI3 K-Akt signal transduction pathway.