葡萄糖调节蛋白78在慢性丙型肝炎患者血清中表达研究

Expression of glucose regulated protein 78 in serum of chronic hepatitis C patients

目的:研究慢性丙型肝炎患者血清葡萄糖调节蛋白78(glucose regulated protein 78kD, GRP78)的表达水平,探讨其与肝损伤程度及病毒复制的关系。方法:随机选取慢性丙型肝炎患者140例,其中轻度74例,中度41例,重度25例,及同期健康者50例。根据病毒载量将患者分为阴性组(HCV-RNA载量<1.0×10^2 copies/ml)、中低拷贝组(1.0×10^2 copies/ml≤HCV-RNA载量<1.0×10^6 copies/ml)、高拷贝组(HCV-RNA载量≥1.0×10^6 copies/ml)。应用ELISA法检测各组血清中GRP78表达水平,探讨其与ALT,RAST, ALB, HCV-Ab, HCV-RNA 的关系。结果:与健康组相比,慢性丙型肝炎患者血清GRP78水平明显增高,但随病程发展逐渐降低(均P<0.05)。GRP78水平与ALT、AST呈显著负相关(r^2=-0.448,P=0.000;r^2=-0.504,P=0.000),与ALB、HCV-Ab则无明显相关(P>0.05)。同时,GRP78水平与病毒复制程度之间差异无统计学意义(P>0.05)。结论:慢性丙型肝炎患者血清中GRP78高表达,且与肝损伤严重程度相关。

Objective:To investigate the expression of glucose regulated proteins 78(GRP78)in chronic hepatitis C(CHC)patients,and discuss the relationship between GRP78 with the degree of liver injury and virus replication.Methods:The GRP78 levels in subjects were measured by ELISA in 140 individuals with CHC and 50 normal healthy persons.Meanwhile,the CHC patients divided into mild,moderate,severe by the degree of liver injury,and negative group(serum HCV-RNA<1.0×10^2 copies/ml),low and medium copy group(1.0×10^2 copies/ml≤Serum HCV-RNA<1.0×10^6 copies/ml),high copy group(serum HCV-RNA≥1.0×10^6 copies/ml).Then,compared the different levels of GRP78 in these groups,and the correlations between GRP78 and serum ALT,AST,ALB,HCV-Ab,HCV-RNA were analyzed.Results:Compared with the healthy group,The level of GRP78 in CHC was significantly higher,and decreased with the development of the disease(all P<0.05).Negative correlation were observed between GRP78 with ALT and AST(r^2=-0.448,P=0.000;r^2=-0.504,P=0.000),and no significant correlation with ALB and HCV-Ab.At the same time,there were no significant differences in GRP78 expression between different viral copies(all P>0.05).Conclusion:The level of GRP78 was significantly increased in CHC and related to the development of disease.