重组HIV-1跨膜蛋白gp41在大肠杆菌中的表达

EXPRESSION OF HUMAN RECOMBINANT HIV-1 gp41 TRANSMEMBRANE PROTEIN IN E.coli

1目的 构建重组 HIV- 1SF2株跨膜蛋白 gp41基因片段的原核表达克隆并在大肠杆菌中表达。2方法 通过聚合酶链反应 (PCR)扩增目的基因 ,然后用限制性内切酶将其与原核表达载体 p MAL - p2定向连接 ,构建成重组质粒转入大肠杆菌 DH5α菌中。经 IPTG诱导 ,SDS- PAGE电泳分析有无重组蛋白表达。3结果 获得了重组的原核表达质粒及其表达产物。4结论 在 p MAL - p2中构建的 HIV - 1gp41重组质粒可在大肠杆菌中表达。

Objective\ To construct the prokaryotic expression plasmid of recombinant HIV 1 gp41 gene fragment of SF2 strain and express in E.coli.DH5α.\ Methods\ The target gene was amplified by PCR ,and the restricted inserting of the target gene into the prokaryotic expression vector pMAL p2 was finished by restricted endonuclease. The constructed recombinant prokaryotic expression plasmid was transformed into E.coli.DH5α., and the inserted gene was then expressed by inducing with IPTG and the prokaryotic expression products were analyzed by SDS PAGE electrophoresis.\ Results\ Recombinant prokaryotic expression plasmid was gained and the prokaryotic expression products of HIV 1 gp41 gene were acquired.\ Conclusion\ The constructed HIV 1 gp41 recombinant plasmid in pMAL p2 could be expressed in E.coli.DH5α. [