百草枯中毒致小鼠肺纤维化模型的建立

Establishment of a mouse lung fibrosis model induced by paraquat poisoning

目的探究百草枯中毒致小鼠肺纤维化模型的建立。方法无特定病原体级别(SPF)的BALB/c小鼠(6~8周龄,雄性,18~22 g)40只,分为A组(空白对照组)、B组(10 mg/kg百草枯单次染毒组)、C组(20 mg/kg百草枯单次染毒组)和D组(隔日1次百草枯染毒组,共3次),采用腹腔注射方式染毒观察至第21天取材,建立小鼠肺纤维化中毒模型。镜下观察组织HE染色变化和马松染色等纤维化改变和不同浓度的生存分析。结果20 mg/kg单次染毒组的小鼠HE染色和马松染色均显示纤维化改变,Ashcroft评分最高,肺组织上皮标志物和间质标志物的蛋白检测均显示有上皮间质改变。结论20 mg/kg腹腔注射单次给药至染毒后21 d的方法建立纤维化模型最好。

Objective To explore the establishment of a model of pulmonary fibrosis in mice induced by paraquat poisoning.Methods Forty BALB/c mice(6~8 weeks old,male,18~22 g)without specific pathogen level(SPF)were divided into group A(blank control group),group B(10 mg/kg single-paraquat exposure group),group C(20 mg/kg single-paraquat exposure group)and group D(one paraquat exposure every other day,three times in total),and the observation was made after intraperitoneal injection for 21 days.The rat lung fibrosis poisoning model was established on the 21st day,and was examined microscopically,and the changes in fibrosis such as HE staining and Masson staining and survival analysis were observed at different concentrations.Results In group C,both HE staining and Masson staining showed fibrotic changes,with the highest Ashcroft score,and protein detection of lung tissue epithelial markers and interstitial markers showed epithelial-mesenchymal changes.Conclusion Single use of 20 mg/kg by intraperitoneal injection is the best in establishing a fibrosis model after 21 d of exposure.