橡胶树炭疽HK-36菌株α-淀粉酶基因家族生物信息学分析

Genome-wide Analysis of theα-Amylase Gene Family in Colletotrichum sp.HK-36 of Rubber Tree

淀粉酶是生物体内参与淀粉水解的一类重要酶,分析橡胶树炭疽菌HK-36菌株α-淀粉酶基因家族的生物学信息可为深入研究炭疽菌α-淀粉酶基因功能打下基础。本研究借助炭疽菌HK-36菌株基因组测序数据,利用生物信息学方法鉴定其α-淀粉酶基因家族,对比分析该菌α-淀粉酶家族成员的基因结构、预测蛋白的理化性质和结构、在炭疽菌亚细胞的定位、启动子元件,构建蛋白三维模型和邻接法进化树。鉴定到5个CgAmys基因,α-淀粉酶家族成员的基因推定的蛋白含有500~549个氨基酸,分别定位于溶酶体、质膜、内质网、过氧物酶体和线粒体中,多为亲水性、酸性蛋白;CgAmys推定蛋白的N端由延长链和螺旋结构组成,C端基本由延长链组成;CgAmys的编码蛋白包含典型的Glyco_hydro_13_cat_dom(IPR006047)结构域,属于糖基水解酶家族13;CgAmys基因启动子上含有大量非生物逆境诱导和激素的顺式作用元件,其中光响应、生长素和茉莉酸诱导有关元件数量最多。橡胶树炭疽菌α-淀粉酶家族基因可能在不同生育期受光照和多种激素调控,并参与炭疽菌对多种逆境的应答调控。该研究有助于进一步解析橡胶树炭疽菌CgAmys基因家族在生长发育及逆境胁迫响应中的功能。

Theα-amylase is an important enzyme involved in starch hydrolysis in organisms.In order to investigate theα-amylase gene family of Colletotrichum sp.(HK-36),the gene family was identified by bioinformatics method based on genome sequencing data of Colletotrichum sp(.HK-36).The gene structure,protein physical and chemical properties,protein structure,subcellular localization,promoter element of theα-amylase gene family of strain HK-36,were analyzed and the three-dimensionalmodel of protein was constructed and the phylogenetic tree of theα-amylases gene family was derived.FiveCgAmys genes of strain HK-36 were identified,which contained 500 to 549 amino acids,located in lysosomes,plasma membrane,endoplasmic reticulum,peroxisome and mitochondria,mostly hydrophilic and acidic proteins.The N-terminal of CgAmys protein was composed of extended chain and spiral structure,and the C-terminal was mainly composed of extended chain.The coding protein of CgAmys contained typical Glyco_hydro_13_cat_dom(IPR006047)domain,belonging to glycosyl hydrolase family 13.The gene promoter contains a large number of abiotic stress inducing and hormone homeopathic elements,amongwhich photoresponse,auxin,jasmonic acid and gibberellin induce related elementswere in themajority,indicating that theα-amy,lase family genes were regulated by light and various hormones at different growth stages.The gene family may be involved in the response to various stresses.The study can help to further analyze the function of the fungal CgAmy gene in the development and stress response.