KAI1、TCF21及PTEN与非小细胞肺癌淋巴结微转移关系的研究

Relationship between KAI1,TCF21,PTEN and Lymph Node Micro-metastasis in Non-small Cell Lung Cancer

目的研究KAI1、TCF21及PTEN与非小细胞肺癌淋巴结微转移的关系。方法回顾性分析本院2005年1月至2010年12月诊治的pN0期非小细胞癌(NSCLC)患者,按5年生存期分为复发组[pN0(+)组]、稳定组[pN0(-)组]。应用CK20mRNA、MUC1mRNA,采用反转录聚合酶链反应(RT-PCR)检测pN0期复发组NSCLC淋巴结微转移情况,应用免疫组化法检测KAI1,TCF21及PTEN在淋巴结中表达情况,并分析其与淋巴结微转移之间关系。结果 56例pN0期NSCLC患者,其中复发组淋巴结微转移患者22例(39.29%),稳定组非淋巴结微转移患者34例(60.71%),且淋巴结微转移患者上述因子KAI1、TCF21及PTEN水平明显低于非淋巴结微转移患者(P <0.05)。结论在NSCLC患者中,KAI1、TCF21及PTEN的表达水平与淋巴结微转移有关,其表达情况可以作为非小细胞肺癌转移及预后的指标。

Objective To analyze the relationship between KAI1,TCF21 and PTEN and lymph node micro-metastasis in non-small cell lung cancer(NSCLC).Methods A retrospective analysis of pN0 stage non-small cell carcinoma(NSCLC)patients diagnosed and treated in our hospital from January 2005 to December 2010 was divided into recurrence group[pN0(+)group]and stable group[pN0(-)group].CK20mRNA and MUC1mRNA were used to detect the micrometastasis of NSCLC lymph nodes in the pN0 stage recurrence group by reverse transcription polymerase chain reaction(RT-PCR),and the expression of KAI1,TCF21 and PTEN in the lymph nodes were detected by immunohistochemistry,and their relationship was analyzed The relationship between lymph node micrometastasis.Results Among 56 patients with pN0 stage NSCLC,22(39.29%)patients with lymph node micrometastasis in the recurrence group,34 patients(60.71%)with non-lymph node micrometastasis in the stable group,and the above-mentioned factors KAI1,TCF21 and PTEN levels were signifi cantly lower in patients with lymph node micrometastasis in patients with non-lymph node micrometastasis(P<0.05).Conclusion The levels of KAI1,TCF21 and PTEN in patients with NSCLC are related to lymph node micro-metastasis of tumor,and could be used as biomarkers to predict the metastasis and prognosis of NSCLC.