miR-665在宫腔粘连及宫颈癌患者中的表达及意义

The expression and clinical significance of miR-665 in intrauterine adhesion and cervical cancer

目的探讨miR-665在宫腔粘连及宫颈癌患者中的表达水平及作用机制,以期为宫腔粘连及宫颈癌患者的早期预防、精准诊疗提供有价值的分子生物学标志物。方法采用TCGA、StarBase等生物信息学软件分析miR-665在宫颈癌患者中的表达水平;采用RT-qPCR实验分析miR-665在宫腔粘连患者组织中的表达水平;采用CCK8实验、集落形成实验、Transwell迁移实验和Western blot实验分析miR-665对hESC细胞增殖能力和迁移能力的影响;采用TargetScan预测和EGFP报告载体实验验证DRAM1为miR-665的靶基因;采用HE染色、免疫组化实验和Western blot实验验证miR-665对体内宫腔粘连的影响。结果miR-665在宫腔粘连患者及宫颈癌患者组织中是低表达的。过表达miR-665能够显著抑制hESC细胞的增殖能力和上皮-间质转化进程。miR-665能够直接靶定DRAM1。过表达miR-665能够发挥缓解体内宫腔粘连的作用。结论miR-665可能通过调控DRAM1在宫腔粘连及宫颈癌中发挥重要作用。

Objective To investigate the expression level and the mechanism of miR-665 in patients of intrauterine adhesion and cervical cancer,and to provide a significative biomarker for the patients of intrauterine adhesion and cervical cancer.Methods TCGA and StarBase were used to detect the expression level of miR-665 in cervical cancer.Real-time quantitative PCR(RT-qPCR)assay was used to detect the expression level of miR-665 in intrauterine adhesion.CCK8 assay,colony formation assay,Trans-well assay and western blot assay were used to the investigate the proliferation and migration of hESC cells.TargetScan prediction and EGFP reporter assay were used to identify the target gene DRAM1 for miR-665.HE staining,immumohistochemical staining(IHC)and Western blot assay were used to investigate the role of miR-665 in the development and progression of intrauterine adhesion.Results miR-665 was down-regulated in patients of IUAs and cervical cancer.It inhibited cell proliferation and EMT process in hESC cells.miR-665 remitted IUAs in vivo by targeting DRAM1.Conclusion miR-665 plays an important role in intrauterine adhesion and cervical cancer through the regulation of DRAM1.