赖氨酸水平对生长猪养分表观消化率和血清氨基酸吸收模式的影响

Effects of lysine levels on nutrient apparent digestibility and serum amino acid absorption patterns in growing pigs

本研究通过两个试验探讨了日粮不同赖氨酸水平对生长猪营养物质消化率及血清氨基酸动态变化的影响。试验1,将96头平均体重为(20.23±1.05)kg的猪分为3组,每组4个重复,每个重复8头,分别饲喂赖氨酸水平为0.6%、0.9%和1.2%的日粮,试验共开展14 d。试验2,在试验1结束的猪中选择36头[每个重复3头,平均体重为(37.68±2.13)kg],饲喂试验1对应的日粮,通过手术在门静脉安装导管进行消化试验。结果:在试验1中,日粮赖氨酸水平显著影响血清大部分氨基酸、尿素氮和总蛋白浓度(P<0.05)。其中,0.6%赖氨酸日粮组总能、干物质、粗蛋白质和磷表观消化率最低(P<0.05)。在试验2中,血清赖氨酸、组氨酸、苯丙氨酸、苏氨酸、缬氨酸、异亮氨酸、甘油三酯和尿素氮浓度受日粮赖氨酸水平显著影响(P<0.05)。此外,大部分血清氨基酸浓度在采食前0.5 h达到最低值,2 h后达到高峰(P<0.05)。在本研究条件下,日粮赖氨酸水平为0.9%时可以使生长猪的营养物质消化率和血清代谢物浓度达到最佳。

Two experiments were conducted to investigate the effects of different dietary lysine levels on nutrient digestibility and serum amino acid dynamics in growing pigs.In experiment 1,96 pigs with an average body weight of(20.23±1.05)kg were divided into 3 groups,4 replicates in each group and 8 replicates in each group were fed diets with lysine levels of 0.6%,0.9%and 1.2%,respectively.The experiment was carried out for 14 days.In experiment 2,36 pigs[3 for each repeat,with an average weight of(37.68±2.13)kg]were selected from the pigs at the end of experiment 1 and fed the diet corresponding to experiment 1.Results:in test 1,the dietary lysine level significantly affected the serum concentration of most amino acids,urea nitrogen and total protein(P<0.05).The total energy,dry matter,crude protein and phosphorus apparent digestibility of the 0.6%lysine diet group were the lowest(P<0.05).In test 2,serum lysine,histidine,phenylalanine,threonine,valine,isoleucine,triglyceride and urea nitrogen concentrations were significantly affected by dietary lysine levels(P<0.05).In addition,the concentration of most serum amino acids reached the minimum value 0.5 h before ingestion and the peak value 2 h later(P<0.05).In this study,the nutrient digestibility and serum metabolite concentration of growing pigs were optimized when the dietary lysine level was 0.9%.