摘要
由于肿瘤的复发和转移,肺癌是一种高度难治的癌症,正成为全球癌症死亡的主要原因之一。过氧化物酶V(Peroxiredoxin V,Prx V)作为过氧化物酶(Peroxiredoxins)家族中的一员,参与肿瘤细胞的形成和发展。研究通过慢病毒载体构建Prx V敲降的A549人非小细胞肺癌细胞系,利用荧光显微镜照相和蛋白免疫印迹法对其结果进行鉴定,通过MTT实验、细胞划痕实验、群落形成实验及蛋白质印迹法检测两种细胞系的细胞增殖、迁移、群落形成能力和相关蛋白的表达水平间的差异。结果表明:敲降Prx V能够有效抑制A549细胞的细胞增殖、迁移以及群落形成能力。研究结果表明Prx V在肺癌细胞的生长调节中发挥重要的作用,为肺癌的治疗研究提供新方向。
Due to tumor recurrence and metastasis,lung cancer was known as a highly refractory cancer and becoming one of the leading causes of cancer death worldwide.Peroxiredoxin V(Prx V),a member of the Peroxiredoxins family,was involved in the form-ation and development of tumor cells.Prx V knockdown A549 human non-small cell lung cancer cell lines were constructed by lentivirus vector.MTT assay,wound healing test,colony-forming assay and western blot were used to detect the differences in cell proliferation,migration,colony-forming ability and expression levels of related proteins between the two cell lines.The results showed that Prx V knockdown could effectively inhibit the proliferation,migration and colony-forming ability of A549 cells,which indicated that Prx V played an important role in the growth regulation of lung cancer cells,providing a new direction for the treatment of lung cancer.
作者
任晨曦
宫伊希
谢丹萍
刘悦
郭晓宇
申贵男
孙虎男
Ren Chenxi;Gong Yixi;Xie Danping;Liu Yue;Guo Xiaoyu;Shen Guinan;Sun Hunan(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)
出处
《黑龙江八一农垦大学学报》
2020年第5期56-62,共7页
journal of heilongjiang bayi agricultural university
基金
黑龙江八一农垦大学创新创业训练计划项目(XC2019026)
黑龙江省自然基金(QC2015121).