一株亚洲象金黄色葡萄球菌的分离鉴定

Isolation,Identification,Pathogenicity of Staphylococcus aureus from Asian Elephant Organs

对西双版纳病死大象送检的脏器进行细菌分离和生化特性分析,经16s rDNA克隆扩增测序后最终确定为金黄色葡萄球菌。该菌在TSA培养基上,菌落呈现浅黄色,生化鉴定表明尿素试验阴性,麦芽糖、乳糖、蔗糖、七叶苷、葡萄糖磷酸盐胨水、鸟氨酸脱羧酶试验阳性。药敏试验发现该菌对阿莫西林、头孢氨苄、林可霉素、青霉素、新生霉素、呋喃唑酮、恩诺沙星、头孢他啶、卡那霉素、四环素、复方新诺明耐药,对环丙沙星、麦迪霉素、利福平、万古霉素高度敏感。并将纯化菌株进行动物回归试验致病性研究,结果显示该菌对小鼠有很强的致病性,病理切片显示心、肾、肠均有出血,肺脏淤血,肝细胞广泛性变性。

We isolated bacteria from organs of a dead Asian elephant(Elephas maximus)from Xishuangbanna,and analyzed their biological characteristics.After the colony,amplification and sequencing of 16s rDNA,one bacterium was identified as Staphylococcus aureus.On TSA medium,its bacterial colony presented pale yellow.Biochemical identification showed that urea tested negative,while maltose,lactose,sucrose,aesculin,glucose phosphate peptone water,ornithine decarboxylase tested positive.Staphylococcus aureus was resistant to amoxicillin,cefalexin,lincomycin,penicillin,neomycin,furazolidone,enrofloxacin,ceftazidime,kanamycin and tetracycline,and was highly sensitive to ciprofloxacin,midecamycin,rifampicin and vancomycin.The purified bacterial strain was highly pathogenic to mice.Pathological sections showed that the heart,kidney,and intestine exhibited haemorrhage,and the lung exhibited congestion.Hepatocytes were widely degenerated.