菖蒲-远志药对组分对Aβ1-42诱导PC12细胞凋亡和钙离子浓度的影响

Influences of Calamus-polygala Medicine on Aβ1-42-induced PC12 Cell Apoptosis and Calcium Ion Concentration

目的观察菖蒲-远志药对组分对β-淀粉样蛋白1-42(Aβ1-42)诱导大鼠肾上腺嗜铬细胞瘤PC12细胞凋亡和钙离子浓度的影响,探讨菖蒲-远志药对组分治疗阿尔茨海默病的作用机制。方法通过Aβ1-42诱导PC12细胞建立阿尔茨海默病细胞模型,分别用α-细辛醚、远志皂苷和α-细辛醚加远志皂苷进行干预,采用AnnexinⅤ/PI双染流式细胞术检测细胞凋亡,采用细胞流式术和钙离子荧光探针fluo3/am检测细胞内钙离子浓度,观察药物干预后各组细胞凋亡率和钙离子浓度。结果各药物干预组细胞凋亡率、钙离子浓度均下降,α-细辛醚组细胞凋亡率、钙离子浓度低于远志皂苷组,α-细辛醚组加远志皂苷组细胞凋亡率、钙离子浓度低于α-细辛醚组、远志皂苷组。结论菖蒲、远志组分具有抗神经细胞凋亡的作用,菖蒲、远志联合应用具有显著增效作用,其抗凋亡作用可能与抑制钙离子有关。

Objective To observe the effect of calamus-polygala medicine on Aβ1-42-induced PC12 cell apoptosis and calcium ion concentration,and to explore the mechanism of calamus-polygala medicine on Alzheimer′s disease.Methods PC12 cells were induced by Aβ1-42 to establish Alzheimer′s disease cell model,and were intervened withα-asarone,polygala saponin,andα-asarone plus polygala saponin,respectively.Cell apoptosis were detected by using AnnexinⅤ/PI double staining flow cytometry.Cell flow cytometry and calcium ion fluorescence probe fluo3/am were used to detect intracellular calcium ion concentration,and the apoptosis rate and calcium ion concentration of each drug intervention group were observed.Results The apoptosis rate and calcium ion concentration of each drug intervention group were decreased.The apoptosis rate and calcium ion concentration ofα-asarone group were lower than those of polygala saponin group,and the cell apoptosis rate,and calcium ion concentration inα-asarone group plus polygala saponin group were lower than those inα-asarone group and polygala saponin group.Conclusion The components of calamus and polygala have anti-apoptotic effects on nerve cells,and the calamus combined with polygala has a significant synergistic effect.The anti-apoptotic effect may be related to the inhibition of calcium ion concentration.