鼻腔给予rhEPO对血管性痴呆大鼠学习记忆能力及视网膜功能的影响

Influences of Recombinant Human Erythropoietin by Intranasal Delivery on Spatial Learning and Memory Capability and Retinal Function in Rats with Vascular Dementia

目的研究重组人促红细胞生成素(rhEPO)对血管性痴呆大鼠的影响。方法选取成年健康雄性无眼疾血管性痴呆大鼠24只,将其按照随机数字表分为假手术组、对照组和实验组。对照组和实验组大鼠结扎双侧颈总动脉(2VO),建立血管性痴呆模型。模型建立3 d后,实验组每周经鼻腔给予150 U rhEPO至建模后8周末;对照组于同一时间鼻腔给予等量生理盐水。8周后应用Morris水迷宫测量3组大鼠的运动及空间学习记忆能力。结果行为学测试结果显示,实验组逃避潜伏期及穿越平台次数均较对照组改善(P<0.05)。与实验组相比,对照组大鼠皮层及海马CA1区锥体神经元数目减少,胞体萎缩、核固缩且皮层厚度变薄,视网膜神经节细胞减少。结论rhEPO能改善血管性痴呆大鼠的学习记忆能力及视网膜功能。

Objective To explore the effects of recombinant human erythropoietin(rhEPO)by intranasal delivery in rats with vascular dementia.Methods Twenty four healthy male vascular dementia SD rats without ocular disease were randomly divided into sham-operated group,control group,and experimental group.The permanent bilateral occlusion of common carotid arteries in SD rats was adopted to established the vascular dementia model in control group and experimental group.The rats in experimental group weekly underwent intranasal delivery at the dose of 150 U rhEPO after chronic ischemia for 3 days,whereas the rats in control group were given equivalent volume of saline at the same time.After 8 weeks,the morris water maze was explored to evaluate the movement and the spatial learning and memory capability.Results The escape latency was shorter and frequency through platform was more in experimental group than those in control group(P<0.05).There were less pyramidal cells and more serious karyopyknosis changes of cerebral cortex and hippocampal CA1 nerve cells and thinner cerebral cortex and fewer retinal ganglion cells in control group.Conclusion rhEPO can improve the ability of memory and spatial orientation and retinal function in rats with vascular dementia.