明胶-羟基磷灰石与明胶-硅羟基磷灰石成骨作用的比较研究

Comparative study on the osteogenesis of gelatin-hydroxyapatite and gelatin-silicon-hydroxyapatite

目的研究无机微量元素硅对明胶-羟基磷灰石(HA)复合材料生物活性的影响。方法本文通过静电纺丝法制备了明胶-羟基磷灰石(HA)和明胶-硅羟基磷灰石(SiHA)复合材料,将样品交联后通过浸泡模拟体液(SBF)来观察Ca-P沉积的矿化行为。在此基础上,共培养小鼠前成骨细胞(MC3T3-E1)和破骨细胞前体细胞(RAW264.7),研究其增殖和分化情况。结果观察扫描电镜发现,明胶-HA样品表面的Ca-P沉积呈现典型的非均匀成核,而明胶-SiHA样品表面则为沿纤维均匀矿化。生物学检测结果表明,明胶-SiHA上(1、3 d)的细胞增殖要明显高于明胶-HA组;第7 d时,差异变得不明显。两种材料表面细胞的分化行为比较,差异无统计学意义。结论在更接近体内环境的共培养条件下,硅掺入明胶-HA材料改变了其表面Ca-P沉积的矿化行为,进而提高了其生物活性。

Objective To investigate the effect of inorganic trace element silicon on the bioactivity of gelatin-hydroxyapatite(HA) composites. Methods Gelatin-hydroxyapatite(HA) and gelatin-silicon-hydroxyapatite(SiHA) membranes were prepared by electrospinning in this study. After cross-linking, the samples were soaked in simulated body fluid(SBF) to observe the mineralization of Ca-P deposition. On this basis, the effects of silicon on cell adhesion, proliferation and differentiation were studied by the co-culture of the mouse precranial bone cells(MC3T3-E1) and leukemia cells of monocyte macrophage(RAW 264.7). Results It was observed by SEM that the Ca-P deposition on the surface of gelatinHA showed typical heterogeneous nucleation while the gelatin-SiHA sample mineralized along the fibers. The biological test results showed that the cell proliferation of gelatin-SiHA sample(day 1 and day 3) was significantly higher than that of gelatin-HA samples, and the difference became insignificant on day 7. The differentiation behavior of cells on the surface of the two materials was not significantly different. Conclusion Therefore, under the condition of co-culture closer to the in vivo environment, the addition of silicon to gelatin-HA material changed the mineralization behavior of Ca-P deposition on its surface, thus promoting its biological activity.