女阴及子宫肿瘤人乳头瘤病毒16/18感染的检测

DETECTION OF HPV 16/18 DNA IN VULVA AND UTERINE NEOPLASMS

1目的 探讨女阴及子宫肿瘤组织人乳头瘤病毒 (HPV) 16 / 18DNA感染及其意义。2方法 应用原位杂交技术对 3个传代的细胞系、96例宫颈标本、75例子宫内膜标本及 33例女阴尖锐湿疣标本 HPV 16 / 18DNA进行了检测。3结果 尖锐湿疣组织 HPV16 / 18DNA感染阳性表达 3例 (9.1% ) ,正常宫颈 2例 (2 0 .0 % )、子宫颈上皮肉瘤变 (CIN ) 5例 (2 7.8% )、CIN 9例 (39.1% )、宫颈鳞癌 2 7例 (6 0 .0 % ) ,4组之间比较差异有极显著性 (χ2 =7.92 3,P<0 .0 1)。2 7例正常子宫内膜组织 HPV16 / 18DNA表达均为阴性 ,48例子宫内膜癌 3例呈阳性 (6 .2 % ) ,其中 1例为腺鳞癌 ,1例为透明细胞癌。4结论 生殖道上皮中存在多种 HPV亚型混合感染 ,子宫内膜癌组织中可见HPV16 / 18感染。

Objective To observe the significance of human papillomavirus(HPV) 16/18 infection in vulva and uterine neoplasms.\ Methods\ 96 cases of cervical specimens and 75 cases of endometrial tissues and 33 cases of vulvar condylomata acuminata were detected for HPV 16/18 DNA by in situ hybridization technic.\ Results\ Positive reaction of HPV 16/18 was found in 3 cases of vulvar condylomata acuminata (9.1%), in 2 cases of normal cervical epithelium(20.0%), 5 cases of CIN Ⅰ( 27.8% ), 9 cases of CIN Ⅲ(39.1) and 27 cases of invasive carcinoma(60.0%), respectively. The difference among these 4 groups was significant( χ 2=7.923, P <0.01). In 27 cases of normal endometrial tissue as control specimen ,no positive reaction was detected. In 48 cases of endometrial carcinoma, 3 cases were positive(6.2%), in which one was adenosquamous carcinoma, one was clear cell carcinoma.\ Conclusion\ There was mixed infection of different subtypes of HPV in genital epithelium. HPV 16/18 infection could be found in endometrial carcinomas. [