一种新型复合碳纳米角电化学生物传感器用于PSA的高灵敏检测

A Novel Coposite Carbon Nanohorns Electrochemical Biosensor for Highly Sensitive Detection of PSA

目的:构建一种基于羧基化碳纳米角与金纳米棒复合物的电化学免疫传感器,并用于前列腺特异性抗原的高灵敏检测。方法:单壁碳纳米角具有卓越的导电率和较高的吸附力。壳聚糖分散的羧基化碳纳米角在电极上成膜并静电吸附半胱氨酸,再利用半胱氨酸吸附羧基化的碳纳米角组装金纳米棒后形成复合材料。固载前列腺抗原抗体,牛血清蛋白封闭非特异性位点,从而制得灵敏度高、稳定性好的前列腺特异性抗原传感器。结果:在最优条件下,免疫传感器用来检测不同浓度的前列腺特异性抗原,其检测的线性范围为0.2-320 ng·L^-1,检出限为0.12 ng·L^-1。结论:该传感器具有良好的选择性,稳定性和重现性,可以用于前列腺癌的早期筛查和诊断。

The objective is to construct an electrochemical immune sensor based on carboxylated carbon nanohorn(CCNH)and gold nanord composites.The method is as follows.Single-welled carbon nanohorns have excellent electrical conductivity and high adsorption.The chitosan-dispersed carboxylated carbon nanohorns form a film on the electrode which electrostatically absorbs cysteine and then absorbs the carboxylated carbon nanohorn with the cysteine to assemble the gold nanoparticles to form a composite material.The prostate antigen antibody is immobilized,and BSA blocks a non-specific site,thereby producing a prostate-specific antigen sensor with high sensitivity and stability.Result shows that under optimal conditions immune sensors were used to detect different concentrations of prostate-specific antigeus,the linear range of detection was 0.2~320ng·L^-1,and the detection limit was 0.12ng·L^-1.It is concluded that the sensor has good selectivity,stability and can be used for early screening and diagnosis of prostate cancer.