比率和比色型硫化氢荧光探针的设计与合成

Design and Synthesis Fluorecent Probe for the Colorimetric and Ratiometric Signaling of Hydrogen Sulfide

基于H2S的生物学意义,本文设计和合成了一种新型的比率和比色型荧光探针CR-DNP,该荧光探针良好的光稳定性,高选择性和出色的灵敏度。发射强度比与NaHS浓度之间存在良好的线性关系,可以定量检测H2S,检测限低至0.4μM。

With recognition of the biological significance of H2S,we have designed and synthesized a novel ratiometric and colorimetric fluorescence probe,CR-DNP based on a H2S induced thiolysis reaction as well as the fluorescence resonance energy transfer process.It displayed some desirable properties,such as good photostability,high selectivity and excellent sensitivity.A good linear relationship between the emission intensity ratios and NaHS concentrations suggested that it can detect H2S quantitatively with a detection limit as low as 0.4μM.