用于病原菌快速和高灵敏检测的电化学方法研究

Study on Electrochemical Method for Rapid and Highly Sensitive Detection of Pathogenic Bacteria

为了检测水样中致病性强的大肠杆菌O157:H7和金黄色葡萄球菌,合成了一种抗菌肽基超顺磁性纳米颗粒,利用抗菌肽和病原菌之间的静电相互作用,将其黏附在病原菌表面。然后利用广泛使用的电化学检测法-循环伏安法(CV),根据细菌浓度和种类的变化引起的电流信号的变化来完成对两种细菌浓度和种类的快速、高效和高灵敏度检测。

In order to detect the pathogenic E.coli O157:H7 and S.aureus in water samples,a kind of superparamagnetic nanoparticles based on antimicrobial peptide was synthesized,which was attached to the surface of pathogens by electrostatic interaction between antimicrobial peptides and pathogens.Then,the widely used electrochemical detection method cyclic voltammetry(CV)was used to detect the concentration and species of bacteria rapidly,efficiently and sensitively according to the current signal changes caused by the changes of bacterial concentration and species.