鸡显性白羽基因PMEL17多态性及基因型鉴定方法

Polymorphism and Genotype Identification of Chicken Dominant White Feather Gene PMEL17

为建立显性白羽基因型检测的快捷方法,试验采用Sanger测序法扫描了白来航、寿光鸡和农大C系前黑素小体蛋白17(PMEL17)基因的全长并进行序列比对,共得到18个与显性白羽表型完全关联的标记。试验选择白来航PMEL17基因第5内含子上的22 bp插入以及第7外显子上的60 bp插入作为显性白羽基因型辅助鉴定的分子标记,并设计了特异性引物对。通过扩大群体和样本数量检测,证明该鉴定方法具有操作简便、结果清晰的优势,检测效力明显提高。研究提供了鸡显性白羽基因型检测的优化位点,为白羽鸡的分子育种提供了新的标记辅助鉴定技术。

To find out a more ideal detection method, this study resequenced the whole pre-melanosomal protein 17(PMEL17) gene of White Leghorn, Shouguang and Nongda C line chicken breeds were obtained by Sanger method,then 18 markers which were fully associated with the dominant white feather phenotype by comparing the sequencing results. The 22 bp insertion mutation in intron 5 and 60 bp insertion in exon 7 of White Leghorn PMEL17 gene were selected as molecular markers for dominant white plumage genotype identification, and specific primers were designed for the two markers.The new methods were proved had the advantages of simple operation and clear results by expanding the sample size testing, and the detection efficiency had been significantly improved. This study provided optimized sites for chicken dominant white feather genotype detection, and provided new marker-assisted identification technology for molecular breeding of white feather chickens.