摘要
本文将来自P.bacterium 1109的甘露糖醇脱氢酶(MDH)表达并提取纯化,研究了该重组酶的酶学性质及其在甘露醇生产中的工艺条件。结果显示重组MDH是一个相对分子量为37 kDa的四聚体。氨基酸序列比对发现其与大多数MDHs的同源性小于40%。该酶的最适pH和温度分别为8.5和80℃,且当金属离子Zn^(2+)存在时,重组MDH的活力提高到对照组的260%。此外,重组MDH在75℃孵育6 h后仍可保留超过85%的残留活性,热稳定性较高,比大多数MDHs的活性高。底物特异性研究表明其对D-果糖具有较高的专一性。重组MDH催化D-果糖的米氏常数(K_m)和催化效率(k_(cat)/K_m)分别为20 mmol/L和7.5 L/(mmol·min)。重组MDH在以400 mmol/L的D-果糖为底物的反应系统中,可将80%以上的D-果糖转化为甘露糖醇。通过对反应条件的优化,为后续工业化生产制备甘露醇奠定了基础。
the mannitol dehydrogenase(MDH)from P.bacterium 1109 was expressed and purified.Enzymatic properties of the recombinant enzyme and the process conditions to produce mannitol were studied.Results showed that recombinant enzyme was a tetramer with molecular weight of 37 kDa.Amino acid sequence alignment showed that its homology with most MDHs was less than 40%.The optimum pH and temperature of the enzyme were 8.5 and 80℃,respectively.The activity of recombinant MDH could increase to 260%of control group with the presence of metal ion Zn2+.In addition,the recombinant MDH could retain more than 85%of residual activity after incubation at 75℃for 6 h,indicating a higher thermal stability than most MDHs.Substrate specificity studies showed that it had a high specificity for D-fructose.The Michaelis constant(Km)and catalytic efficiency(kcat/Km)of D-fructose catalyzed by recombinant MDH were 20 mmol/L and 7.5 L/(mmol·min),respectively.The recombinant MDH could produce more than 80%mannitol in the reaction system composed of 400 mmol/L of D-fructose substrate.The optimization of the reaction conditions laid the foundation for the subsequent industrial production of mannitol.
作者
逯付之
徐炜
吴昊
张文立
光翠娥
沐万孟
LU Fu-zhi;XU Wei;WU Hao;ZHANG Wen-li;GUANG Cui-e;MU Wan-meng(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China)
出处
《食品工业科技》
CAS
北大核心
2020年第19期137-143,165,共8页
Science and Technology of Food Industry
基金
国家自然基金青年基金(31801583)
江苏省自然科学青年基金(BK20180607)
江南大学基本科研计划青年基金(JUSRP11966)。