摘要
目的:基于模式动物秀丽线虫探究羧甲基壳聚糖的抗衰老能力。方法:检测羧甲基壳聚糖处理前后,秀丽虫寿命、运动能力、吞咽频率以及排泄周期的变化,同时测定过氧化氢酶和谷胱甘肽过氧化物酶的活力,判断羧甲基壳聚糖线虫体内抗氧化的能力。结果:500和1000 mg/L羧甲基壳聚糖分别能显著(P<0.05)和极显著(P<0.01)延长秀丽线虫的寿命,而500 mg/L能够极显著(P<0.01)提高秀丽线虫运动能力活跃度,加速咽泵运动,缩短排泄周期。同时,100、500和1000 mg/L羧甲基壳聚糖均能极显著(P<0.01)提高秀丽线虫过氧化氢酶和谷胱甘肽过氧化物酶的活力。结论:500 mg/L的羧甲基壳聚糖抗衰老作用较好强,具有抗氧化、抗衰老的应用前景。
Objective:To investigate the anti-aging activity of carboxymethyl chitosan in Caenorhabditis elegans.Method:The lifespan,locomotion behavior,swallowing frequency and defecation cycle of C.elegans before and after treatment with carboxymethyl chitosan were detected.Moreover,the superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)of C.elegans were detected.Result:500,1000 mg/L carboxymethyl chitosan could significantly(P<0.05,P<0.01)prolong the life of caenorhabditis elegans,while 500 mg/L could significantly(P<0.01)improve the movement of Caenorhabditis elegans,accelerate the movement of pharyngeal pump,shorten the excretion period.At the same time,100,500 and 1000 mg/L carboxymethyl chitosan could significantly increase the activity of catalase and glutathione peroxidase in caenorhabditis elegans(P<0.01).Conclusion:500 mg/L carboxymethyl chitosan has a significant anti-aging effect and has application prospects of anti-oxidation and anti-aging.
作者
李卓航
杨敏
张锋伦
龚祝南
马世宏
束成杰
LI Zhuo-hang;YANG Min;ZHANG Feng-lun;GONG Zhu-nan;MA Shi-hong;SHU Cheng-jie(College of Food and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China;China Nanjing Institute for Comprehensive Utilization of Wild Plants,Nanjing 211100,China;Nanjing Jiangning District Hospital of Traditional Chinese Medicine,Nanjing 211100,China)
出处
《食品工业科技》
CAS
北大核心
2020年第19期327-331,共5页
Science and Technology of Food Industry
基金
新疆维吾尔自治区重大科技专项(2016A03006)。
关键词
羧甲基壳聚糖
抗衰老
抗氧化
体内
秀丽线虫
carboxymethyl chitosan
anti-aging
antioxidant
in vivo
Caenorhabditis elegans