牛OPU卵子体外受精和早期胚胎培养的方法研究

Methodology on in vitro Fertilization of Bovine OPU Ovum and EarlyEmbryo Culture

为了提高牛OPU卵子来源的胚胎生产效率,本试验对牛活体采卵获得的卵子分别用不同系列的培养液培养,用不同精子离心方法及不同的脱颗粒细胞方法进行操作。通过卵裂率和桑囊率的结果,以确定培养液及各种操作方法的可行性。结果表明,进口成品培养液的桑囊率虽高于自配溶液(34.29±9.20%vs 26.45±20.01%),但差异不显著,不同的精子离心方法和扒卵方法也没有显著差异。通过本研究可以为源于牛OPU卵子的体外胚胎生产程序的优化提供参考。

In order to increase the efficiency of bovine embryo production derived from OPU ovum,in this experiment,the oocytes obtained from bovine ovum pick-up were cultured in different series of culture medium,and were operated by different sperm centrifugal methods and different zygote-stripping methods.By comparing the results of the cleavage rate and development rate of morulae and blastocysts,the feasibility of the culture medium and various operation methods was determined.The results showed that development rate of morulae and blastocysts cultured with the imported medium was higher than that of the self-preparation medium(34.29±9.20%vs26.45±20.01%),but there was no significant difference between the two serials of medium.There was no significant difference between different sperm centrifugal methods and zygote-stripping methods,either.This study can provide some reference for optimizing the procedure of bovine in vitro embryo production system derived from ovum pick-up.