西宁市牛衣原体病流行病学调查

An Epidemiological Survey of Chlamydia Bovis in Xining City

为了摸清西宁市牛衣原体的感染情况,本研究采集青海省西宁市三县三区221个养殖场(户)的牛血液样品、肛拭子和生殖道拭子各1255份,采用间接血凝(IHA)试验和PCR试验进行了牛衣原体感染情况调查,结果显示,间接血凝试验阳性检出率为1.51%(19/1255)、肛拭子PCR和生殖道拭子PCR阳性检出率均与间接血凝试验一致,表明西宁地区牛存在不同程度的衣原体感染。

In order to find out the infection of Chlamydia bovis in Xining city,indirect hemagglutination(IHA)test and PCR test were used to investigate the positive rate and infection of Chlamydia bovis.Totally 1255 samples of bovine blood,anal swabs and genital swabs were collected from 221 farmers(households)in three counties and three districts of Xining City,Qinghai Province.The results showed that the positive rate of indirect hemagglutination test was 1.51%.The positive rates of(19/1255),anal swab PCR and genital swab PCR were consistent with those of indirect hemagglutination test,indicating that there were different degrees of Chlamydia infection in cattle raised in Xining area.