基于“药效-成分分析”的银屑灵优化方2号方抗银屑病配伍规律探讨

Discussion on the synergy rule of PSORI-CM02 in treating psoriasis based on pharmacodynamic-component analysis

目的:探讨银屑灵优化方2号方(PSORI-CM02)治疗银屑病的配伍规律。方法:将PSORI-CM02拆分为清热解毒方、凉血活血方和清热解毒+凉血活血方,采用心得安豚鼠银屑病模型,考察总方及拆方对该模型的病理状态影响并进行Baker评分;利用HPLC测定总方及拆方中绿原酸、芍药苷、落新妇苷、黄杞苷、迷迭香酸、白藜芦醇在水提液中的含量变化。结果:PSORI-CM02及拆方均能够显著降低银屑病豚鼠的Baker评分(P<0.01),PSORI-CM02药效显著优于凉血活血组(P<0.05)和清热解毒组(P<0.01),其余组间无显著差异;落新妇苷和黄杞苷含量与处方药效存在正相关关系。结论:配伍通过增强处方活性成分溶出增强药效。

Objective:To investigate the possible synergy rule of PSORI-CM02 in the treating psoriasis.Methods:PSORI-CM02 was divided into clearing heat and detoxification decoction,cooling blood and invigorating circulation decoction,and the combination of above decoction.Psoriasis-like model in guinea pigs were applied as the pharmacological study model,and pathological morphology assessment was examined with baker score.The content of chlorogenic acid,paeoniflorin,astilbin,engeletin,rosmarinic acid and resveratrol in different dismantlement of PSORI-CM02 were determined by high performance liquid chromatography.Results:Both dismantling prescription and PSORI-CM02 decreased the baker scores in psoriasis-like model of guinea pigs(P<0.01).The effect of PSORI-CM02 was superior to the cooling blood and invigorating circulation group(P<0.05)and the clearing heat and detoxification group(P<0.01),and there was no significant difference in other medicated groups.There is a positive correlation between the content of astilbin and engeletin and the efficacy of the prescription.Conclusion:By enhancing the dissolution of prescription active ingredients,the synergy enhanced the effect of medicine.