磷脂酶D在枯草芽胞杆菌中表达及酶法催化合成磷脂酰丝氨酸的研究

Expression of Phospholipase D in Bacillus subtilis and Enzymatic Synthesis of Phosphatidylserine

磷脂酰丝氨酸(PS)因具备改善记忆、缓解精神压力等作用,常被应用于功能性食品和药物生产中.相比于提取法,以磷脂酶D(PLD)催化转酯反应合成PS具有更为环境友好、安全高效、易于操作等优点.本研究利用枯草芽胞杆菌表达并分泌来源于郝氏链霉菌(Streptomyces halstedii)TCCC21102的PLD,转酯酶活力达2.53 U/mL;经酶学性质分析表明,其最适温度为40℃、最适pH为5.5;利用该PLD在构建的单水相体系催化大豆磷脂酰胆碱(PC)合成PS,当反应24 h时PS产率达到最高,为42.6%.本研究为酶法制备新食品原料PS,实现其在功能性食品和制药行业中的应用奠定了基础.

Phosphatidylserine(PS)is often used in functional foods and medicines because of its positive effects on improving memory and relieving mental stress.Compared with the extraction method,phosphatidyl acylation catalyzed by phospholipase D(PLD)has more advantages,as the process is environment friendly,safer,more efficient,and ease to operate.In this study,Bacillus subtilis was used to efficiently express and secrete PLD Streptomyces halstedii TCCC21102,and the transphosphatidylation activity of PLD reached 2.53 U/mL.The PLD showed the highest activity at 40℃and pH 5.5.The yield of PS was up to 42.6%after 24 h reaction catalyzed by PLD when soybean phosphatidylcholine(PC)was used as the substrate.PS production in aqueous water system has a good application prospect in functional food and pharmaceutical industry.