荧光染色法在真菌镜检中的应用

Application of fluorescence staining in microscopic examination of fungi

目的探讨荧光染色法在真菌镜检中的应用价值。方法收集350例不同来源的临床标本,直接涂片后分别进行革兰染色和荧光染色镜检,同时接种沙保罗培养基进行真菌培养鉴定,3种方法任意一种为阳性,则认为该标本为真菌检测阳性,并以此为标准来计算3种方法的检出率、灵敏度和阴性预测值,应用χ2检验评价3种方法的差异。结果350例临床标本应用荧光染色法、革兰染色法和真菌培养鉴定法的检出率分别为53.4%、11.4%和43.4%,灵敏度分别为86.6%、18.5%和70.4%,阴性预测值分别为82.2%,43.2%和67.7%,各组数据比较,差异均有统计学意义(P<0.05)。结论荧光染色法在真菌检测中显示出一定的优势,值得推广应用。

Objective To explore the application value of fluorescent staining method in fungal microscopic examination.Methods A total of 350 clinical samples from different sources were collected and conducted the Gram staining and fluorescence staining microscopic examination after direct smear.At the same time,inoculating Sabouraud medium to identify fungi.The fungi detection was defined as positive if the results in any method among 3 kinds of method presented positive.With this criterion,the detection rate,sensitivity and negative predictive value were calculated and the Chi-square test was used to evaluate the difference among these three methods.Results In 350 clinical samples,the fungal detection rates of fluorescent staining,Gram staining and fungal culture were 53.4%,11.4%and 43.4%respectively,the sensitivities were 86.6%,18.5%and 70.4%respectively,the negative predictive values were 82.2%,43.2%and 67.7%,respectively,the data differences among various groups were statistically significant(P<0.05).Conclusion The fluorescent staining method presents a certain superiority in the fungal detection and is worth to be promoted and applied.