结直肠癌组织中CD133及LGR5蛋白的表达及差异表达基因分析

Analysis on expression of CD133 and LGR5 protein and differential expressed genes in colorectal cancer tissues

目的研究结直肠癌组织CD133和LGR5蛋白以及差异表达基因的表达情况。方法采用免疫组化法检测正常结直肠、结直肠息肉、结直肠癌组织中CD133和LGR5蛋白表达水平。采用生物信息学手段分析结直肠癌细胞转录组数据,进行差异表达基因筛选、GO分析及KEGG分析,分析CD133及LGR5基因在结直肠癌、大肠癌及结直肠癌伴肝转移细胞中的表达情况,以及结直肠癌患者预后生存率。结果与正常结直肠组织和息肉组织比较,结直肠癌组织中CD133及LGR5蛋白的表达均明显升高(P<0.05)。分析GSE2509数据库的转录组表达谱共筛选到1265个差异表达基因,其中,与结肠癌SW480细胞系相比,CD133和LGR5基因在强侵袭性的结肠癌SW620细胞系中均呈高表达。分析GSE21510的转录表达谱,与正常对照相比,结直肠癌细胞的CD133及LGR5基因亦呈高表达。对GSE72718数据库的表达谱数据进行分析,比较原发性结直肠癌细胞以及结直肠癌伴肝转移细胞的表达谱,其中CD133基因差异有统计学意义(P<0.05),LGR5基因差异无统计学意义(P>0.05)。对GSE12945数据库中结直肠癌肿瘤组织表达谱进行分析,以及对患者进行生存分析,发现CD133和LGR5基因表达水平与结直肠癌患者总体生存率无关(P>0.05)。结论CD133和LGR5蛋白在结直肠癌组织中高表达,与肿瘤侵袭性相关。

Objective To investigate the expressions of colorectal cancer tissue CD133,LGR5 protein and differential expressed genes.Methods The expression levels of CD133 and LGR5 protein in normal colorectal,colorectal polyps and colorectal cancer tissues were detected by adopting the immunohistochemical method.The transcription group data of colorectal cancer cells were analyzed by adopting the bioinformatic measures,and the differentially expressed gene(DEGs)screening,GO analysis and KEGG analysis were performed.The expression of CD133 and LGR5 gene in the cells of colorectal cancer,colon cancer cells and colorectal cancer complicating liver metastasis and the prognostic survival rate in the patients with colorectal cancer were analyzed.Results Compared with the normal colorectal tissue and polyps tissue,the expression of CD133 and LGR5 protein in the colorectal cancer tissues was increased significantly(P<0.05).A total of 1265 differentially expressed gene were screened out by analyzing the expression spectrum of the transcriptome in the GSE2509 database,in which compared with colorectal cancer cell line SW480,CD133 and LGR5 gene showed high expression in the strong invasive colon cancer cell line SW620.In the transcription expression spectrum analysis of GSE21510,compared with the normal control group,CD133 and LGR5 gene of colorectal cancer cells also showed the high expression.In the expression spectrum data analysis of GSE72718 database,compared with the expression spectrum of primary colorectal cancer cells and colorectal cancer cells complicating liver metastasis,the CD133 gene difference had statistical significance(P<0.05),while the LGR5 gene difference had no statistical significance(P>0.05).The analysis of expression spectrum data in GSE72718 database and patients survival analysis found expression level of CD133 and LGR5 gene had no relation with the overall survival rate of the patients with colorectal cancer(P>0.05).Conclusion CD133 and LGR5 are highly expressed in colorectal cancer tissue,which is correlated with tumor invasiveness.