HPLC法同时测定复方龙胆碳酸氢钠片中3种龙胆特征性成分的含量

Simultaneous Determination of Three Characteristic Components of Gentiama in Compound Gentian and Sodium Bicarbonate Tablets by HPLC

目的:建立HPLC法同时测定复方龙胆碳酸氢钠片中马钱苷酸、龙胆苦苷、獐牙菜苷3种龙胆特征性成分的含量。方法:采用HPLC法,色谱柱:Techmate C18柱(250 mm×4.6 mm,5μm);流动相:0.1%磷酸溶液-乙腈,梯度洗脱;检测波长:240 nm;柱温:35℃;流速:1.0 ml·min^-1;进样量:20μl。结果:马钱苷酸、龙胆苦苷、獐牙菜苷线性浓度范围分别为0.302~120.873μg·ml^-1(r=0.9999),0.302~120.926μg·ml^-1(r=0.9998),0.275~110.152μg·ml-1(r=0.9999),加样回收率分别为98.48%(RSD=1.2%,n=6),98.06%(RSD=0.9%,n=6),95.61%(RSD=0.9%,n=6)。结论:该方法专属性强,准确性好,灵敏度高,可用于同时测定复方龙胆碳酸氢钠片中3种龙胆特征性成分。

Objective:To establish a method for the simultaneous determination of 3 characteristic components of Gentiama in compound gentian and sodium bicarbonate tablets.Methods:An HPLC method was adopted.The determination was performed on a Techmate C18 column(4.6 mm×250 mm,5μm)with the mobile phase consisting of acetonitrile-0.1%phosphate acid solution(gradient elution)at the flow rate of 1.0 ml·min^-1.The detection wavelength was 240nm and the column temperature was 35℃.The sample size was 20μl.Results:The linear range of loganic acid,gentiopicroside and sweroside was 0.302-120.873μg·ml-1(r=0.9999),0.302-120.926μg·ml-1(r=0.9998)and 0.275-110.152μg·ml^-1(r=0.9999),respectively.The recoveries were 98.48%(RSD=1.2%,n=6),98.06%(RSD=0.9%,n=6)and 95.61%(RSD=0.9%,n=6),respectively.Conclusion:The method is simple,precise,stable and reproducible,which can be used for the simultaneous determination of 3 characteristic components of Gentiama in compound gentian and sodium bicarbonate tablets.