穴位埋线对局灶性脑缺血再灌注损伤大鼠运动功能的影响

Effects of Acupoint Catgut Embedding on Motor Function in Cerebral Ischemia-reperfusion Injury in Rats

目的研究穴位埋线对局灶性脑缺血再灌注大鼠运动功能的影响,并探讨其可能作用机制。方法取3月龄健康雄性SPF级Sprague-Dawley大鼠48只,随机分为3组,每组16只。假手术组仅离断右侧颈外动脉,模型组和穴位埋线组制作局灶性脑缺血90 min再灌注大鼠模型,穴位埋线组缺血再灌注90 min后于百会穴、右侧顶颞前斜线进行穴位埋线。再灌注1 d、3 d、7 d、14 d后,进行神经功能评分和网屏实验;再灌注14 d后,TTC染色比较各组脑梗死体积;HE染色观察神经细胞损伤;免疫组织化学、Western blotting和逆转录实时定量聚合酶链反应(RT-qPCR)检测缺血半暗带脑组织内胶质纤维酸性蛋白(GFAP)、突触素I的表达。结果与假手术组相比,模型组神经功能评分显著降低(t>11.851,P<0.001),网屏实验时间显著缩短(t>6.190,P<0.001),GFAP阳性表达增加(P<0.05),突触素I阳性表达下降(P<0.05);与模型组比较,穴位埋线组神经功能评分显著增加(t>3.464,P<0.001),网屏实验时间显著增加(t>3.642,P<0.001),脑梗死体积显著减小(F=93.426,P<0.001),GFAP阳性表达降低(P<0.05),突触素I阳性表达增加(P<0.05)。结论穴位埋线可促进局灶性脑缺血再灌注损伤大鼠运动功能恢复,可能与抑制星形胶质细胞反应性增生,增加缺血半暗带内轴突的再生,加强突触间连接与传导有关。

Objectives To investigate the effect of acupoint catgut embedding on motor function of rats with cerebral ischemia-reperfusion,and explore its possible mechanism.Methods A total of 48 three-month old healthy specified pathogen free male Sprague-Dawley rats were randomly divided into three groups with 16 rats in each group.The right external carotid artery was severed only in sham operation group.Right middle cerebral artery occlusion was induced for 90 minutes under isoflurane anesthesia using a monofilament in model group and acupoint catgut embedding group.Ninety minutes after reperfusion,the acupoint catgut embedding group accepted acupoint catgut embedding on Baihui(DU20)acupoint and anterior oblique line of vertex-temporal.After one,three,seven and 14 days of reperfusion,they were assessed with Neurological Evaluation and Screen Prehensile Test.After 14 days of reperfusion,the infarct volume was measured with TTC staining;the expression of glial fibrillary acidic protein(GFAP)and Synapsin I was detected with immunohistochemistry,Western blotting and reverse transcription real-time quantitative polymerase chain reaction(RT-qPCR).Results Compared with the sham operation group,the neurological score significantly decreased(t>11.851,P<0.001),the prehensile time significantly decreased(t>6.190,P<0.001),the expression of GFAP increased(P<0.05),and the expression of Synapsin I decreased(P<0.05)in the model group.Compared with the model group,the neurological score significantly increased(t>3.464,P<0.001),the prehensile time significantly increased(t>3.642,P<0.001),the cerebral infarction volume significantly decreased(F=93.426,P<0.001),the expression of GFAP decreased(P<0.05),and the expression of Synapsin I increased(P<0.05)in the acupoint catgut embedding group.Conclusion Acupoint catgut embedding could promote the recovery of motor function of rats with cerebral ischemia-reperfusion injury,which may be related with inhibiting the proliferation of astrocytes,increasing the regeneration of axons in the ischemic penumbra and enhancing synaptic connection.