自动化神经组织单细胞悬液制备探索性实验教学实践

Exploratory Experimental Teaching Practice on Automatic Neural Tissue Dissociation to Viable Single Cells

组织单细胞悬液制备是细胞生物学中的基础技能,常用的组织解离方法有机械法和酶法,不同解离方法在细胞得率和细胞活性上差异较大。在神经科学的各个研究领域,包括发育研究、疾病病理生理学、药物和毒素筛选,以及神经疾病动物模型的移植等均需要使用分离的单细胞。然而神经组织细胞间紧密的黏附性及轴突和树突的易碎性阻碍单细胞的获得。自动化组织处理器结合机械法与酶法双重作用可获得高质量的细胞悬液。利用这一技术开展自主设计型神经组织单细胞悬液制备实验,以提升学生综合分析应用能力,拓展对前沿科学技术的认识。通过科研与教学相融合的方式,更好地激发医学生的学习热情,提高医学生的科研素质,实现研究型与创新型人才培养的目标。

Single cell suspension preparation is a basic skill in Cell Biology.Commonly tissues are dissociated by mechanical and enzymatic methods.Different dissociation methods vary greatly in cell yield and cell viability.Neural single cell suspensions are in active use for a myriad of research applications,including the study of development,disease pathophysiology,drug and toxin screening,and grafting in animal models of neurological disorders.However,the acquisition of neural single cells is hampered due to the tight adhesion between neurons and the fragility of axons and dendrites.Automatic tissue dissociator combines the mechanical and enzymatic methods and can obtain high-quality cell suspensions.This technology is used to carry out a self-designed experiment of neural single cell suspension preparation,which is helpful to enhance students’ comprehensive analysis and application capabilities,and expand their understanding of cutting-edge science and technology.The integration of scientific research and teaching can better stimulate the learning enthusiasm of medical students,improve their scientific research quality,and achieve the goal of culticating research-oriented and innovative talents.